Breast cancer subtype specific biochemical responses to radiation
| Autor: | Pedro H. B. Aoki, Andrew Jirasek, Julian J. Lum, Phiranuphon Meksiarun, Alexandre G. Brolo, Regivaldo G. Sobral-Filho, Samantha J. Van Nest |
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| Rok vydání: | 2018 |
| Předmět: |
Receptor
ErbB-2 medicine.medical_treatment Breast Neoplasms Radiation Biology Spectrum Analysis Raman 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor Nucleic Acids Electrochemistry medicine Environmental Chemistry Humans External beam radiotherapy skin and connective tissue diseases Receptor Spectroscopy Principal Component Analysis 010401 analytical chemistry Cancer Discriminant Analysis Proteins medicine.disease Lipids Epithelium 0104 chemical sciences medicine.anatomical_structure Ki-67 Antigen Cell culture 030220 oncology & carcinogenesis Nucleic acid Cancer research Female Glycogen |
| Zdroj: | The Analyst. 143(16) |
| ISSN: | 1364-5528 |
| Popis: | External beam radiotherapy is a common form of treatment for breast cancer. Among patients and across different breast cancer subtypes, the response to radiation is heterogeneous. Radiation-induced biochemical changes were examined by Raman spectroscopy using cell lines that represent a spectrum of human breast cancer. Principal component analysis (PCA) and partial least squares discriminant analysis (PLSDA) revealed unique Raman spectral features in the HER2 and Ki67 subtype. The changes in Raman spectral profiles to different doses of radiation (0-50 Gy) included variations in the levels of proteins, lipids, nucleic acids and glycogen. Importantly, the differences in radiation-induced changes on the normal breast epithelial cell line MCF10A could be discriminated within and across the various breast tumor cell lines. These results demonstrate a novel approach to uncover differences between breast cancer cell subtypes and surrounding normal tissues by their biochemical variations in response to radiation. |
| Databáze: | OpenAIRE |
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