The effect of nerve growth factor on dispersed neuronal hela heterokaryons
| Autor: | Gere DiZerega, John Morrow |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Cell type
Density gradient Chick Embryo HeLa Developmental Neuroscience Culture Techniques Ganglia Spinal Botany medicine Centrifugation Density Gradient Animals Microscopy Interference Nerve Growth Factors Growth Substances Heterokaryon Neurons biology fungi biology.organism_classification Sendai virus Trypsinization Cell biology Nerve growth factor medicine.anatomical_structure Neurology Neuron Spinal Nerve Roots HeLa Cells |
| Zdroj: | Experimental neurology. 28(2) |
| ISSN: | 0014-4886 |
| Popis: | Dorsal root ganglia from 7-day chick embryos were minced and slowly trypsinized to produce a suspension of cells. This suspension was centrifuged in an albumin density gradient and the fraction enriched with neuronal perikarya collected. This fraction was combined with an equivalent number of HeLa cells. The two cell types are easily identifiable with interference microscopy. The two cell types were fused by the addition of β-propiolactone-inactivated Sendai virus. The resultant heterokaryons were cultured in modified hanging drop preparations in the presence of nerve growth factor and supplemented medium. Nerve growth factor stimulated the growth of long processes from the neuronal-HeLa heterokaryons when added to both long- and short-term cultures thereby demonstrating retention by the heterokaryon of a differentiated property of the neuron. We conclude that differentiated properties can be retained in somatic cell hybrids. |
| Databáze: | OpenAIRE |
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