Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
| Autor: | Sueli A. Clara, Vania dos Santos Nunes, Sandro José Conde, Maria Teresa De Sibio, Célia Regina Nogueira, Edson Nacib Jorge, Renata de Azevedo Melo Luvizotto, Gláucia Maria Ferreira da Silva Mazeto, Miriane de Oliveira, Sarah Santiloni Cury |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Ciência e Tecnologia do Estado de São Paulo (IFSP) |
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Endocrinology Diabetes and Metabolism Retinoic acid lcsh:Medicine Inflammation Severity of Illness Index lcsh:Diseases of the endocrine glands. Clinical endocrinology Dexamethasone Graves' ophthalmopathy chemistry.chemical_compound Adipocyte Internal medicine Receptors retinoic acid Humans Medicine Retinoid Fibroblast Glucocorticoids lcsh:RC648-665 business.industry Retinoic Acid Receptor alpha lcsh:R graves ophthalmopathy Graves ophthalmopathy Fibroblasts medicine.disease eye diseases Endocrinology medicine.anatomical_structure chemistry gene expression Gene expression medicine.symptom business Orbit hormones hormone substitutes and hormone antagonists Glucocorticoid medicine.drug |
| Zdroj: | Archives of Endocrinology and Metabolism, Vol 62, Iss 3, Pp 366-369 (2018) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2359-4292 2359-3997 |
| DOI: | 10.20945/2359-3997000000044 |
| Popis: | Made available in DSpace on 2018-12-11T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01. Added 1 bitstream(s) on 2019-10-09T18:31:56Z : No. of bitstreams: 1 S2359-39972018000300366.pdf: 123932 bytes, checksum: 884f7b3ea5771ca0dc08ec15ac8f5036 (MD5) Made available in DSpace on 2018-12-11T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01. Added 1 bitstream(s) on 2019-10-09T18:31:56Z : No. of bitstreams: 1 S2359-39972018000300366.pdf: 123932 bytes, checksum: 884f7b3ea5771ca0dc08ec15ac8f5036 (MD5) Made available in DSpace on 2018-12-11T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01. Added 1 bitstream(s) on 2019-10-09T18:31:56Z : No. of bitstreams: 1 S2359-39972018000300366.pdf: 123932 bytes, checksum: 884f7b3ea5771ca0dc08ec15ac8f5036 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) Instituto Federal de Educação Ciência e Tecnologia do Estado de São Paulo (IFSP) Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) FAPESP: #:03/03651-0 FAPESP: 2011/18464-8 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|