Physiological characteristics of chemostatically grownCitrobacter freundii as a function of the specific growth rate and type of nutrient limitation
| Autor: | F. Kaprálek, J Dolezal |
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| Rok vydání: | 1976 |
| Předmět: |
Population
Chemostat Microbiology Ammonium Chloride Adenosine Triphosphate Citrobacter Enterobacteriaceae Growth rate Energy charge Pyruvates education education.field_of_study biology Chemistry Galactose General Medicine biology.organism_classification Adenosine Monophosphate Aerobiosis Dilution Citrobacter freundii Adenosine Diphosphate Glucose Biochemistry Steady state (chemistry) Energy Metabolism Energy source |
| Zdroj: | Folia Microbiologica. 21:168-177 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/bf02883152 |
| Popis: | Citrobacter freundii was grown aerobically in a chemostat on a mineral medium with galactose or glucose as carbon and energy sources under limitation by carbon or nitrogen source respectively. At various specific growth rates ranging from 7 to 95% mumax the culture in steady state was analysed and growth yield, specific metabolic rate of substrate utilization, intracellular concentration of pyruvate, ATP, ADP, AMP and energy charge were determined and plotted as functions of dilution rate. In all four types of experiments the physiological state of cells remained practically independent of dilution rate up to D=0.6 mumax, and at a given specific growth rate nearly independent on mumax and type of limitation. At approximately D=0.6 mumax, which is close to the maximum output dilution rate Dm, the physiological state of the cells changed: growth yields decreased and intr cellular pyruvate and adenylates concentrations increased. Consequently, in a given medium two dilution rates exist at which growth rate dx/dt is the same but the physiology of the population is quite different. |
| Databáze: | OpenAIRE |
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