Cytoskeletal tension actively sustains the migratory T cell synaptic contact
| Autor: | Erin Janssen, Darrell J. Irvine, Mira Tohme, Yeh Chuin Poh, Michael Mak, Mariane B. Melo, Sudha Kumari, Nicki Watson, Raif S. Geha, Michael L. Dustin |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Chemistry T cell macromolecular substances Cell junction Immunological Synapses Cell biology Synaptic contact 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Myosin medicine Antigen-presenting cell Cytoskeleton Actin 030304 developmental biology 030215 immunology |
| DOI: | 10.1101/437236 |
| Popis: | SummaryWhen migratory T cells encounter antigen presenting cells (APCs), they arrest and form radially symmetric, stable intercellular junctions termed immunological synapses which facilitate exchange of crucial biochemical information and are critical for T cell immunity. While the cellular processes underlying synapse formation have been well-characterized, those that maintain the symmetry, and thereby the stability of the synapse remain unknown. Here we identify an antigen-triggered mechanism that actively promotes T cell synapse symmetry by generating cytoskeletal tension in the plane of the synapse through focal nucleation of actin via Wiskott -Aldrich syndrome Protein (WASP), and contraction of the resultant actin filaments by myosin II. Following T cell activation, WASP is degraded, leading to cytoskeletal rearrangement and tension decay, which result in synapse breaking. Thus, our study identifies and characterizes a mechanical program within otherwise highly motile T cells that sustains the symmetry and stability of the T cell-APC synaptic contact. |
| Databáze: | OpenAIRE |
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