Novel binding partners of Ldb1 are required for haematopoietic development
| Autor: | Roger Patient, Jeroen Krijgsveld, Frank Grosveld, Martin Gering, Arnd Hostert, Natalia Meier, Patrick Rodriguez, Maria Chiara Monti, John Strouboulis, Sanja Krpic |
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| Přispěvatelé: | Biomoleculaire Massaspectrometrie, Massaspectrometrie, Dep Scheikunde, Cell biology, Meier, N, Krpic, S, Rodriguez, P, Strouboulis, J, Monti, Maria, Krijgsveld, J, Gering, M, Patient, R, Hostert, A, Grosveld, F. |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
LMO2
Chromatin Immunoprecipitation Embryo Nonmammalian Repressor Transcription factor complex Transfection Farmacie/Biofarmaceutische wetenschappen (FARM) Mice Transcription Factor 4 Erythroid Cells Cell Line Tumor Animals Erythropoiesis Molecular Biology Zebrafish Adaptor Proteins Signal Transducing Homeodomain Proteins Genetics biology Farmacie(FARM) Nuclear Proteins Cell Differentiation GATA1 Cell Biology Hematology LIM Domain Proteins Zebrafish Proteins Embryo Mammalian Hematopoietic Stem Cells biology.organism_classification Cyclin-Dependent Kinase 9 Cell biology DNA-Binding Proteins Repressor Proteins Haematopoiesis Molecular Medicine Leukemia Erythroblastic Acute Stem cell Protein Binding Transcription Factors Developmental Biology TAL1 |
| Zdroj: | Development, 133(24), 4913-4924. Company of Biologists Ltd |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Ldb1, a ubiquitously expressed LIM domain binding protein, is essential in a number of tissues during development. It interacts with Gata1, Tal1, E2A and Lmo2 to form a transcription factor complex regulating late erythroid genes. We identify a number of novel Ldb1 interacting proteins in erythroleukaemic cells, in particular the repressor protein Eto-2 (and its family member Mtgr1), the cyclin-dependent kinase Cdk9, and the bridging factor Lmo4. MO-mediated knockdowns in zebrafish show these factors to be essential for definitive haematopoiesis. In accordance with the zebrafish results these factors are coexpressed in prehaematopoietic cells of the early mouse embryo,although we originally identified the complex in late erythroid cells. Based on the change in subcellullar localisation of Eto-2 we postulate that it plays a central role in the transition from the migration and expansion phase of the prehaematopoietic cells to the establishment of definitive haematopoietic stem cells. |
| Databáze: | OpenAIRE |
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