Correction: Genetic diversity and population structure of indigenous chicken in Rwanda using microsatellite markers

Autor: Pauline Assami, Tobias Otieno Okeno, Christian Tiambo Keambou, Nasser Yao, Kiplangat Ngeno, Kizito Nishimwe, Anique Ahou Gbotto, Sylvere Mboumba, Janvier Mahoro, Richard Habimana
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Veterinary medicine
Heredity
Population structure
Population genetics
Breeding
Poultry
Microsatellite Loci
Loss of heterozygosity
Geographical Locations
Genotype
Gamefowl
education.field_of_study
Multidisciplinary
Heterozygosity
Ecology
Eukaryota
Population Surveillance
Vertebrates
Microsatellite
Medicine
Inbreeding
Research Article
Ecological Metrics
Science
Population
Locus (genetics)
Biology
Analysis of molecular variance
Indigenous
Birds
Gene Types
Genetics
Humans
Animals
Allele
education
Alleles
Genetic diversity
Evolutionary Biology
Population Biology
Ecology and Environmental Sciences
Organisms
Rwanda
Correction
Genetic Variation
Biology and Life Sciences
Species Diversity
Evolutionary biology
Fowl
Genetic Loci
Amniotes
People and Places
Africa
Genetic Polymorphism
Chickens
Population Genetics
Microsatellite Repeats
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 4, p e0225084 (2020)
PLoS ONE, Vol 15, Iss 9, p e0238966 (2020)
ISSN: 1932-6203
Popis: Rwanda has about 4.5 million of indigenous chicken (IC) that are very low in productivity. To initiate any genetic improvement programme, IC needs to be accurately characterized. The key purpose of this study was to ascertain the genetic diversity of IC in Rwanda using microsatellite markers. Blood samples of IC sampled from 5 agro-ecological zones were collected from which DNA was extracted, amplified by PCR and genotyped using 28 microsatellite markers. A total of 325 (313 indigenous and 12 exotic) chicken were genotyped and revealed a total number of 305 alleles varying between 2 and 22 with a mean of 10.89 per locus. 186 distinct alleles and 60 private alleles were also observed. The frequency of private alleles was highest in samples from the Eastern region, whereas those from the North West had the lowest. The influx of genes was lower in the Eastern agro-ecological zone than the North West. The mean observed heterozygosity was 0.6155, whereas the average expected heterozygosity was 0.688. The overall inbreeding coefficient among the population was 0.040. Divergence from the Hardy-Weinberg equilibrium was significant in 90% of loci in all the populations. The analysis of molecular variance revealed that about 92% of the total variation originated from variation within populations. Additionally, the study demonstrated that IC in Rwanda could be clustered into four gene groups. In conclusion, there was considerable genetic diversity in IC in Rwanda, which represents a crucial genetic resource that can be conserved or optimized through genetic improvement.
Databáze: OpenAIRE
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