Substrate preference of Bifidobacterium adolescentis MB 239: compared growth on single and mixed carbohydrates
| Autor: | Alberto Amaretti, Maddalena Rossi, Diego Matteuzzi, Elena Tamburini, Giuseppe Vaccari, Anna Pompei, Tatiana Bernardi, Simona Zanoni |
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| Přispěvatelé: | A.Amaretti, E.Tamburini, T.Bernardi, A.Pompei, S.Zanoni, G.Vaccari, D.Matteuzzi, M.Rossi |
| Rok vydání: | 2006 |
| Předmět: |
Glycoside Hydrolases
Chemostat Applied Microbiology and Biotechnology chemistry.chemical_compound Hydrolysis Monosaccharide Glycosyl Biomass Raffinose Lactose chemistry.chemical_classification chemostat Growth medium Bifidobacterium prebiotics sugar mixtures glycosyl hydrolases HPTLC-AMD Fructose General Medicine Culture Media chemistry Biochemistry Carbohydrate Metabolism Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 73:654-662 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0500-9 |
| Popis: | The utilization of mono-, di-, and oligosaccharides by Bifidobacterium adolescentis MB 239 was investigated. Raffinose, fructooligosaccharides (FOS), lactose, and the monomeric moieties glucose and fructose were used. To establish a hierarchy of sugars preference, the kinetics of growth and sugar consumption were determined on individual and mixed carbohydrates. On single carbon sources, higher specific growth rates and cell yields were attained on di- and oligosaccharides compared to monosaccharides. Analysis of the carbohydrates in steady-state chemostat cultures, growing at the same dilution rate on FOS, lactose, or raffinose, showed that monomeric units and hydrolysis products were present. In chemostat cultures on individual carbohydrates, B. adolescentis MB 239 simultaneously displayed alpha-galactosidase, beta-galactosidase, and beta-fructofuranosidase activities on all the sugars, including monosaccharides. Glycosyl hydrolytic activities were found in cytosol, cell surface, and growth medium. Batch experiments on mixtures of carbohydrates showed that they were co-metabolized by B. adolescentis MB 239, even if different disappearance kinetics were registered. When mono-, di-, and oligosaccharides were simultaneously present in the medium, no precedence for monosaccharides utilization was observed, and di- and oligosaccharides were consumed before their constitutive moieties. |
| Databáze: | OpenAIRE |
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