High-density lipoproteins reduce the neutralizing effect of hepatitis C virus (HCV)-infected patient antibodies by promoting HCV entry
| Autor: | Jean Dubuisson, Marlène Dreux, Anne Op De Beeck, Pauline Horellou, François-Loïc Cosset, Thierry Gustot, Cécile Voisset, Gilles Duverlie, Ngoc Vu-Dac |
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| Přispěvatelé: | Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Virologie moléculaire (HIV-BLV-HTLV) (ULB126), Université libre de Bruxelles (ULB), Virologie humaine, École normale supérieure - Lyon (ENS Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Unité de Virologie clinique et fondamentale (UVCF), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, This work was supported by grants from the Agence Nationale de Recherche sur le Sida et les hépatites virales (ANRS), INSERM (ATC ‘hépatite C’) and the Association pour la Recherche sur le Cancer (ARC). C. V. was supported by an ANRS post-doctoral fellowship. J. D. is an international scholar of the Howard Hughes Medical Institute., We thank Czeslaw Wychowski and David Delgrange for their help with the HCVcc system. We also thank Angéline Bilheu, Sophana Ung and André Pillez for excellent technical assistance and Birke Bartosch and Laurence Cocquerel for critical reading of the manuscript. We also thank J. McKeating and T. Wakita for the gift of reagents., Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Hepacivirus
Hepatitis C virus In Vitro Techniques medicine.disease_cause Neutralization Virus MESH: Hepatitis C Antibodies/immunology 03 medical and health sciences Flaviviridae MESH: Hepatitis C/virology 0302 clinical medicine Neutralization Tests Virology MESH: Antibodies Monoclonal/immunology MESH: Hepacivirus/physiology medicine Humans Scavenger receptor 030304 developmental biology MESH: In Vitro Techniques 0303 health sciences MESH: Hepatitis C/immunology MESH: Humans biology MESH: Hepacivirus/pathogenicity MESH: Neutralization Tests Antibodies Monoclonal Hepatitis C Antibodies Scavenger Receptors Class B biology.organism_classification MESH: Lipoproteins HDL/physiology Hepatitis C 3. Good health Immunology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology biology.protein MESH: Hepatitis C/blood MESH: Lipoproteins HDL/immunology 030211 gastroenterology & hepatology Antibody Lipoproteins HDL Lipoprotein MESH: Scavenger Receptors Class B/physiology |
| Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 2006, 87 (9), pp.2577-2581. ⟨10.1099/vir.0.81932-0⟩ Journal of General Virology, 2006, 87 (9), pp.2577-2581. ⟨10.1099/vir.0.81932-0⟩ |
| ISSN: | 0022-1317 1465-2099 |
| DOI: | 10.1099/vir.0.81932-0⟩ |
| Popis: | The neutralizing activity of anti-hepatitis C virus (HCV) antibodies is attenuated by a factor present in human sera, which has been proposed to be high-density lipoproteins (HDLs). HDLs have also been shown to facilitate the entry of HCV pseudoparticles (HCVpp) into target cells. Here, the aim of the study was to determine whether HDL-mediated facilitation of HCVpp and infectious HCV (HCVcc) entry and attenuation of neutralization are two related phenomena. The data indicated that HDLs attenuate neutralization at a constant rate. In addition, as for HDL-mediated facilitation of HCVpp entry, attenuation of neutralization depended on the expression of the scavenger receptor BI (SR-BI) and its selective lipid-uptake function. Finally, kinetic experiments showed that HDL-mediated facilitation of HCVpp entry is more rapid than virus neutralization. Altogether, these observations indicate that HCV is exploiting the physiological activity of SR-BI for promoting its entry into target cells, which consequently also protects the virus against neutralizing antibodies. |
| Databáze: | OpenAIRE |
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