A Modified Technique for Combination of Cytochemistry and Radioautography of Haemic Cells
| Autor: | Haakon Breien Benestad |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Vinyl Chloride Bone Marrow Cells Mice Inbred Strains Azure Stains Monocytes Giemsa stain law.invention Diffusion Mice law medicine Animals Humans Cells Cultured Blood Cells Chromatography Staining and Labeling Human blood Histocytochemistry Chemistry Modified technique Membranes Artificial Hematology Photographic fixer Dichloroethylenes medicine.anatomical_structure Peroxidases Cytochemical staining Cytochemistry Autoradiography Bone marrow |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1977.tb01477.x |
| Popis: | Cultured mouse bone marrow and human blood cells were labelled with 3H-thymidine, smeared, and stained, using peroxydase reagents or the May-Grünwald Giemsa procedure. The slides were dipped in a approximately 12% solution of polyvinylchloride, bleached by a photographic fixer and processed for liquid emulsion radioautography. This method yielded technically very satisfactory radioautographs of pre-stained smears. |
| Databáze: | OpenAIRE |
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