Combination of conventional blood cultures and the SeptiFast molecular test in patients with suspected sepsis for the identification of bloodstream pathogens
| Autor: | Maria Grazia Milia, Emilpaolo Manno, Francesco Cerutti, Elisa Burdino, Gabriella Gregori, Francesco Giuseppe De Rosa, Valeria Ghisetti, Rosangela Milano, Giovanni Di Perri, Tiziano Allice, Pietro Caramello, T. Ruggiero |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Adult Microbiological Techniques Pathology medicine.medical_specialty Polymicrobial infection medicine.drug_class Antibiotics Gastroenterology Sensitivity and Specificity Sepsis Bloodstream infection Internal medicine medicine Humans Blood culture In patient Combined method High prevalence medicine.diagnostic_test Bacteria business.industry Fungi General Medicine medicine.disease Infectious Diseases Blood Molecular Diagnostic Techniques business |
| Popis: | We evaluated performances of the molecular test Septi Fast (SF) for the detection of agents of bloodstream infection (BSI) in patients with suspected sepsis, the majority of them under antibiotic treatment and at high prevalence of HIV-1 infection (10.5%). Matched SF and blood culture (BC) samples (n=1186) from 1024 patients were studied. Two hundred fifty-one episodes of BSI out of 1144 were identified with the combined methods (22%). SF identified more episodes of BSI than BC: 206 versus 176 (χ 2 =7.008, P =0.0081) and a significantly higher number of Gram-negative bacteria than BC (77 versus 53, χ 2 =9.12; P =0.0025), as well as of polymicrobial infections (χ 2 =4.50, P =0.0339). In conclusion, SF combined with BC improved the diagnosis of sepsis, especially in immunocompromised patients. |
| Databáze: | OpenAIRE |
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