Non-Enzymatic Hydrolysis of RNA in Workers of the AntNylanderia pubens
| Autor: | Steven M. Valles, Charles A. Strong, David H. Oi, Eileen A. Buss |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
RNA Stability
RNase P medicine.medical_treatment Ethylenediaminetetraacetic acid Polymerase Chain Reaction Article chemistry.chemical_compound medicine Animals nucleic acid degradation Ovum Protease Invasive species Nylanderia pubens biology Ants Hydrolysis fungi Pupa RNA General Medicine Microfluidic Analytical Techniques biology.organism_classification Molecular biology ANT Biochemistry chemistry Spectrophotometry RNA purification Larva Insect Science Female RNA extraction |
| Zdroj: | Journal of Insect Science |
| ISSN: | 0970-3837 1536-2442 |
| Popis: | During preparation of total RNA from Nylanderia pubens (Forel) (Hymenoptera: Formicidae) workers for use in expression library construction, severe RNA degradation consistently occurred. This degradation was masked by spectrophotometric analysis but clearly evident by microfluidic-based assay. Although not specifically identified, the degrading entity was endogenous and localized to the abdomen (terminal abdominal segments) of adult ants. RNA degradation was not observed in preparations of larvae, non-melanized pupae, or eggs. Various RNase and protease inhibitors had no protective effect. However, the metal chelating agent ethylenediaminetetraacetic acid prevented RNA degradation and provides insight into the occurrence. |
| Databáze: | OpenAIRE |
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