Erythrosin B: a versatile colorimetric and fluorescent vital dye for bacteria
| Autor: | Greg A Perry, Erin E Eberhard, Ann L. Braverman, Alison M Cunningham, Josef D. Franke |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Bacteriological Techniques
0303 health sciences Bacteria biology medicine.diagnostic_test 030306 microbiology Cell Membrane Flow Cytometry biology.organism_classification Fluorescence General Biochemistry Genetics and Molecular Biology Flow cytometry 03 medical and health sciences Erythrosine Biochemistry medicine Erythrosin B Colorimetry Viability assay Fluorescent Dyes 030304 developmental biology Biotechnology |
| Zdroj: | BioTechniques. 68:7-13 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2019-0066 |
| Popis: | Rapidly assaying cell viability for diverse bacteria species is not always straightforward. In eukaryotes, cell viability is often determined using colorimetric dyes; however, such dyes have not been identified for bacteria. We screened different dyes and found that erythrosin B (EB), a visibly red dye with fluorescent properties, functions as a vital dye for many Gram-positive and -negative bacteria. EB worked at a similar concentration for all bacteria studied and incubations were as short as 5 min. Given EB's spectral properties, diverse experimental approaches are possible to rapidly visualize and/or quantitate dead bacterial cells in a population. As the first broadly applicable colorimetric viability dye for bacteria, EB provides a cost-effective alternative for researchers in academia and industry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|