Effects of Intraperitoneal Administration of Mifepristone on Glucocorticoid Status of Experimental Animals
| Autor: | O. P. Cherkasova, O. I. Kuz’mina, N. A. Palchikova, V. G. Selyatitskaya, N. V. Kuznetsova |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty 030209 endocrinology & metabolism General Biochemistry Genetics and Molecular Biology Excretion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glucocorticoid receptor Blood serum Hormone Antagonists Corticosterone Internal medicine 11-beta-Hydroxysteroid Dehydrogenase Type 1 Adrenal Glands medicine Animals Chronic stress Rats Wistar Glucocorticoids General Medicine Mifepristone Endocrinology chemistry Liver Pregnenolone 030217 neurology & neurosurgery Glucocorticoid Injections Intraperitoneal medicine.drug |
| Zdroj: | Bulletin of experimental biology and medicine. 161(2) |
| ISSN: | 1573-8221 |
| Popis: | We studied the content of corticosterone and its precursors in the adrenal glands, corticosterone in blood serum and daily urine of rats, and activity of first and second isoforms of 11β-hydroxysteroid dehydrogenase in the liver and kidneys of rats after 15 daily intraperitoneal injections of 0.9% NaCl or glucocorticoid receptor blocker mifepristone in 0.9% NaCl. Daily injections of NaCl reduced the levels of pregnenolone, progesterone, and corticosterone in the adrenal glands, increased corticosterone excretion with urine, enhanced activity of the first isoform of 11β-hydroxysteroid dehydrogenase in the liver and reduction in activity of the second isoform of this enzyme in the kidneys. These changes are typical manifestations of chronic stress. Mifepristone restored pregnenolone content in the adrenal glands and increase in corticosterone concentration in the blood. Under these conditions, activity of the first isoform of 11β-hydroxysteroid dehydrogenase in the liver did not change, and a decrease in activity of the second isoform of the enzyme in the kidneys was less pronounced. The results suggest that mifepristone abolished the stress-mediated increase in activity of the first isoform of 11β-hydroxysteroid dehydrogenase in the liver and reduced local production of glucocorticoid hormones and their metabolic effects in hepatocytes. |
| Databáze: | OpenAIRE |
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