Amplification and overexpression of epidermal growth factor receptor gene in human oropharyngeal cancer
| Autor: | Vikram Sanghavi, Rama Nair, Dhananjaya Saranath, Ashok R. Mehta, R. G. Panchal, Madhav G. Deo |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Cancer Research India Malignancy Epidermal growth factor Gene expression Gene duplication medicine Humans RNA Messenger Epidermal growth factor receptor Gene Aged biology Gene Amplification Cancer Middle Aged medicine.disease Primary tumor ErbB Receptors Gene Expression Regulation Neoplastic Blotting Southern Oropharyngeal Neoplasms Oncology Carcinoma Squamous Cell Cancer research biology.protein Female |
| Zdroj: | European Journal of Cancer Part B: Oral Oncology. 28:139-143 |
| ISSN: | 0964-1955 |
| DOI: | 10.1016/0964-1955(92)90043-z |
| Popis: | The presence of epidermal growth factor receptor (EGF-R) gene was determined in 84 patients with squamous cell carcinoma (SCC) of the oropharyngeal region—a highly prevalent, chewing-tobacco associated malignancy in India, using Southern hybridisation analysis of DNA extracted from primary tumor tissues. We observed a 3- to 8-fold amplification of EGF-R gene in 19 66 (29%) of the SCCs of the oral cavity, and about 30-fold EGF-R amplification in 2 18 (11%) hypopharyngeal cancers. Dot blot analysis of total RNA from several tumour tissues, revealed overexpression of the EGF-R gene in the examined patients, with the EGF-R gene amplified. 4 patients with single copy EGF-R gene, did not exhibit overexpression of the gene. Within our sample set, no correlation was evident between EGF-R gene amplification and clinico-pathological parameters of the malignancy. The amplification and overexpression of the EGF-R gene observed in the primary tumour tissues of 25% ( 21 84 ) of the human oropharyngeal cancers, indicate possible involvement of the gene in the pathogenesis of these cancers. |
| Databáze: | OpenAIRE |
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