Expression of Neuropeptide FF Defines a Population of Excitatory Interneurons in the Superficial Dorsal Horn of the Mouse Spinal Cord that Respond to Noxious and Pruritic Stimuli
| Autor: | Andrew M. Bell, Erika Polgár, Andrew J. Todd, Masahiko Watanabe, Maria Gutierrez-Mecinas |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
substance P ERK extracellular signal-regulated kinases neurotensin CCK cholecystokinin Substance P pERK phospho-ERK chemistry.chemical_compound 0302 clinical medicine Gastrin-releasing peptide Neuropeptide FF Extracellular Signal-Regulated MAP Kinases Neurons education.field_of_study gastrin releasing peptide General Neuroscience LSN lateral spinal nucleus NKB neurokinin B cholecystokinin Posterior Horn Cells medicine.anatomical_structure Gastrin-Releasing Peptide Spinal Cord eGFP enhanced green fluorescent protein Excitatory postsynaptic potential LPb lateral parabrachial area Neurokinin B PKCγ protein kinase Cγ isoform Oligopeptides Spinal Cord Dorsal Horn CTb cholera toxin B subunit Population Green Fluorescent Proteins Neuropeptide Mice Transgenic Biology Article neurokinin B 03 medical and health sciences GRP gastrin releasing peptide Interneurons NPFF neuropeptide FF medicine Animals education ALT anterolateral tract Spinal cord 030104 developmental biology chemistry nervous system Neuroscience NPFF 030217 neurology & neurosurgery DAPI 4′ 6-diamidino-2-phenylindole |
| Zdroj: | Neuroscience |
| ISSN: | 1873-7544 0306-4522 |
| Popis: | The great majority of neurons in the superficial dorsal horn of the spinal cord are excitatory interneurons, and these are required for the normal perception of pain and itch. We have previously identified 5 largely non-overlapping populations among these cells, based on the expression of four different neuropeptides (cholecystokinin, neurotensin, neurokinin B and substance P) and of green fluorescent protein driven by the promoter for gastrin-releasing peptide (GRP) in a transgenic mouse line. Another peptide (neuropeptide FF, NPFF) has been identified among the excitatory neurons, and here we have used an antibody against the NPFF precursor (pro-NPFF) and a probe that recognises Npff mRNA to identify and characterise these cells. We show that they are all excitatory interneurons, and are separate from the five populations listed above, accounting for ~ 6% of the excitatory neurons in laminae I-II. By examining phosphorylation of extracellular signal-regulated kinases, we show that the NPFF cells can respond to different types of noxious and pruritic stimulus. Ablation of somatostatin-expressing dorsal horn neurons has been shown to result in a dramatic reduction in mechanical pain sensitivity, while somatostatin released from these neurons is thought to contribute to itch. Since the great majority of the NPFF cells co-expressed somatostatin, these cells may play a role in the perception of pain and itch. Highlights • NPFF is expressed by around 6% of the excitatory interneurons in the superficial dorsal horn of the mouse spinal cord. • NPFF cells differ from those that express substance P, cholecystokinin, neurotensin or neurokinin B. • Although some NPFF cells express gastrin-releasing peptide (GRP), they do not express GFP in a GRP-GFP mouse line. • Some NPFF cells are activated by noxious or pruritic stimuli. |
| Databáze: | OpenAIRE |
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