Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates
| Autor: | Ying-Hsiang Wang, Pei-Yu Huang, Chih-Cheng Lu, I-An Tsai, Che-Chun Chen, Chishih Chu, Hung Ming Chen |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Serotype medicine.drug_class 030106 microbiology Antibiotics Taiwan Erythromycin Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology Streptococcus agalactiae Fish Diseases 03 medical and health sciences Streptococcus agalactiae (GBS) Drug Resistance Multiple Bacterial Streptococcal Infections Drug Resistance Bacterial medicine Animals Humans Pathogen Pulsotype Clindamycin Streptococcosis Virology Anti-Bacterial Agents Penicillin 030104 developmental biology Multilocus sequence typing Female Research Article MLST Tilapia medicine.drug |
| Zdroj: | BMC Microbiology |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-016-0794-4 |
| Popis: | Background Streptococcus agalactiae (GBS) is a common pathogen to infect newborn, woman, the elderly, and immuno-compromised human and fish. 37 fish isolates and 554 human isolates of the GBS in 2007–2012 were investigated in serotypes, antibiotic susceptibility, genetic difference and pathogenicity to tilapia. Results PCR serotyping determined serotype Ia for all fish GBS isolates and only in 3.2 % (3–4.2 %) human isolates. For fish isolates, all consisted a plasmid less than 6 kb and belonged to ST7 type, which includes mainly pulsotypes I and Ia, with a difference in a deletion at the largest DNA fragment. These fish isolates were susceptible to all antimicrobials tested in 2007 and increased in non-susceptibility to penicillin, and resistance to clindamycin and ceftriaxone in 2011. Differing in pulsotype and lacking plasmid from fish isolates, human serotype Ia isolates were separated into eight pulsotypes II–IX. Main clone ST23 included pulsotypes II and IIa (50 %) and ST483 consisted of pulsotype III. Human serotype Ia isolates were all susceptible to ceftriaxone and penicillin and few were resistant to erythromycin, azithromycin, clindamycin, levofloxacin and moxifloxacine with the resistant rate of 20 % or less. Using tilapia to analyze the pathogenesis, fish isolates could cause more severe symptoms, including hemorrhage of the pectoral fin, hemorrhage of the gill, and viscous black and common scites, and mortality (>95 % for pulsotype I) than the human isolates ( |
| Databáze: | OpenAIRE |
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