A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival*
Autor: | Janel R. Beckley, Junmin Peng, Jun-Song Chen, Yanling Yang, Kathleen L. Gould |
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Rok vydání: | 2015 |
Předmět: |
Protein domain
Proteomics Cell morphology Biochemistry Substrate Specificity Analytical Chemistry Deubiquitinating enzyme Ubiquitin Endopeptidases Schizosaccharomyces Humans Molecular Biology Conserved Sequence biology Research Cell Membrane Cell Polarity biology.organism_classification Transport protein Cell biology Protein Transport Schizosaccharomyces pombe biology.protein Schizosaccharomyces pombe Proteins |
Zdroj: | Molecular & Cellular Proteomics. 14:3132-3141 |
ISSN: | 1535-9476 |
Popis: | Deubiquitinating enzymes (DUBs), cysteine or metallo- proteases that cleave ubiquitin chains or protein conjugates, are present in nearly every cellular compartment, with overlapping protein domain structure, localization, and functions. We discovered a cohort of DUBs that are involved in membrane trafficking (ubp4, ubp5, ubp9, ubp15, and sst2) and found that loss of all five of these DUBs but not loss of any combination of four, significantly impacted cell viability in the fission yeast Schizosaccharomyces pombe (1). Here, we delineate the collective and individual functions and activities of these five conserved DUBs using comparative proteomics, biochemistry, and microscopy. We find these five DUBs are degenerate rather than redundant at the levels of cell morphology, substrate selectivity, ubiquitin chain specificity, and cell viability under stress. These studies reveal the complexity of interplay among these enzymes, providing a foundation for understanding DUB biology and providing another example of how cells utilize degeneracy to improve survival. |
Databáze: | OpenAIRE |
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