Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis
Autor: | Shujing Liu, Lars Hennig, Rafael Muñoz-Viana, Minerva S. Trejo-Arellano, Lejon E. M. Kralemann, Claudia Köhler |
---|---|
Přispěvatelé: | Swedish Research Council, Knut and Alice Wallenberg Foundation, Swedish University of Agricultural Sciences |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Ubiquitin-Specific Proteases lcsh:QH426-470 H3K27me3 Arabidopsis H2Aub macromolecular substances DUB 01 natural sciences 03 medical and health sciences LHP1 Genetics (medical genetics to be 30107 and agricultural genetics to be 40402) Gene Expression Regulation Plant REF6 Monoubiquitination Gene Silencing lcsh:QH301-705.5 Psychological repression 030304 developmental biology Polycomb Repressive Complex 1 0303 health sciences biology Arabidopsis Proteins Research Polycomb Repressive Complex 2 H2AK121ub Responsiveness biology.organism_classification PRC2 PRC1 EMF1 Cell biology lcsh:Genetics Histone lcsh:Biology (General) biology.protein Demethylase Developmental Biology 010606 plant biology & botany |
Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Genome Biology, Vol 21, Iss 1, Pp 1-19 (2020) Genome Biology |
ISSN: | 1474-760X |
Popis: | [Background]: Stable gene repression is essential for normal growth and development. Polycomb repressive complexes 1 and 2 (PRC1&2) are involved in this process by establishing monoubiquitination of histone 2A (H2Aub1) and subsequent trimethylation of lysine 27 of histone 3 (H3K27me3). Previous work proposed that H2Aub1 removal by the ubiquitin-specific proteases 12 and 13 (UBP12 and UBP13) is part of the repressive PRC1&2 system, but its functional role remains elusive. [Results]: We show that UBP12 and UBP13 work together with PRC1, PRC2, and EMF1 to repress genes involved in stimulus response. We find that PRC1-mediated H2Aub1 is associated with gene responsiveness, and its repressive function requires PRC2 recruitment. We further show that the requirement of PRC1 for PRC2 recruitment depends on the initial expression status of genes. Lastly, we demonstrate that removal of H2Aub1 by UBP12/13 prevents loss of H3K27me3, consistent with our finding that the H3K27me3 demethylase REF6 is positively associated with H2Aub1. [Conclusions]: Our data allow us to propose a model in which deposition of H2Aub1 permits genes to switch between repression and activation by H3K27me3 deposition and removal. Removal of H2Aub1 by UBP12/13 is required to achieve stable PRC2-mediated repression. This work was supported by the Swedish Research Council VR (621-2014-5822 to LH) and the Knut and Alice Wallenberg Foundation (2012.0087 to LH). Open access funding provided by Swedish University of Agricultural Sciences. |
Databáze: | OpenAIRE |
Externí odkaz: |