Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony
| Autor: | Dema Ali, Salwa Bdour, Nour Sharar, Abdalla Awidi, Malak Alzubide, Dana Alhattab, Hanan Jafar |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Keratinocytes
QH301-705.5 Cellular differentiation Biology Stem cell marker Catalysis Article Flow cytometry Inorganic Chemistry stem cells medicine Humans Physical and Theoretical Chemistry Biology (General) Clonogenic assay Cell adhesion Molecular Biology QD1-999 Spectroscopy Cells Cultured medicine.diagnostic_test integumentary system Gene Expression Profiling keratinocyte stem cells differentiated cells Organic Chemistry Cell Differentiation General Medicine Computer Science Applications Cell biology Clone Cells transit amplifying cells Chemistry medicine.anatomical_structure Epidermal Cells Gene Expression Regulation Gene chip analysis holoclone Stem cell Keratinocyte paraclone meroclone Biomarkers |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 10810, p 10810 (2021) International Journal of Molecular Sciences Volume 22 Issue 19 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | The stemness in keratinocyte stem cells (KSCs) is determined by their gene expression patterns. KSCs are crucial in maintaining epidermal homeostasis and wound repair and are widely used candidates for therapeutic applications. Although several studies have reported their positive identifiers, unique biomarkers for KSCs remain elusive. Here, we aim to identify potential candidate stem cell markers. Human epidermal keratinocytes (HEKs) from neonatal foreskin tissues were isolated and cultured. Single-cell clonal analysis identified and characterized three types of cells: KSCs (holoclones), transient amplifying cells (TACs meroclones), and differentiated cells (DSCs paraclones). The clonogenic potential of KSCs demonstrated the highest proliferation potential of KSCs, followed by TACs and DSCs, respectively. Whole-transcriptome analysis using microarray technology unraveled the molecular signatures of these cells. These results were validated by quantitative real-time polymerase chain reaction and flow cytometry analysis. A total of 301 signature upregulated and 149 downregulated differentially expressed genes (DEGs) were identified in the KSCs, compared to TACs and DSCs. Furthermore, DEG analyses revealed new sets of genes related to cell proliferation, cell adhesion, surface makers, and regulatory factors. In conclusion, this study provides a useful source of information for the identification of potential SC-specific candidate markers. |
| Databáze: | OpenAIRE |
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