Long Non-Coding RNA TFAP2A-AS1 Inhibits Cell Proliferation and Invasion in Breast Cancer via miR-933/SMAD2
| Autor: | Bin Zhou, Jinhai Tang, Haiyan Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Cell cycle checkpoint Carcinogenesis Cell Apoptosis Breast Neoplasms Smad2 Protein 030204 cardiovascular system & hematology Biology medicine.disease_cause Mice 03 medical and health sciences 0302 clinical medicine Lab/In Vitro Research Cell Line Tumor microRNA medicine Animals Humans Neoplasm Invasiveness 3' Untranslated Regions Aged Cell Proliferation Mice Inbred BALB C Cell growth RNA Cell Cycle Checkpoints General Medicine Middle Aged Cell cycle Prognosis MicroRNAs medicine.anatomical_structure Transcription Factor AP-2 Cell culture 030220 oncology & carcinogenesis MCF-7 Cells Cancer research Heterografts Female RNA Long Noncoding |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND It is well documented that long non-coding RNAs (lncRNAs) are involved in the progression of multiple human tumors by sponging microRNAs (miRNAs). However, whether lncRNA TFAP2A-AS1 plays a role in the tumorigenesis of breast cancer (BC) remains undetermined. MATERIAL AND METHODS Real-time PCR (qRT-PCR) assay was performed to detect the relative mRNA expression of TFAP2A-AS1 and miR-933. Flow cytometry analysis, CCK-8 assay, and Transwell assay were applied to detect the effects of TFAP2A-AS1 overexpression on cell cycle, apoptosis, viability, and invasion of BC cells. In vivo proliferation assay was performed to evaluate the effects of TFAP2A-AS1 overexpression on tumor growth. Bioinformatics methods, dual-luciferase reporter, RNA immunoprecipitation (RIP), and RNA pull-down assays were used to predict and validate the interaction between TFAP2A-AS1 and miR-933, as well as SMAD2 and miR-933. Western blot assay was performed to examine the protein expression of SMAD2 in treated BC cells. RESULTS TFAP2A-AS1 expression was significantly lower in BC tissues and cell lines, and patients with high TFAP2A-AS1 expression exhibited a better prognosis than those with low TFAP2A-AS1 expression. Overexpression of TFAP2A-AS1 in BC cells caused cell cycle arrest, promoted cell apoptosis, suppressed cell ability, and attenuated cell invasion in vitro, and inhibited tumor growth in vivo. TFAP2A-AS1 was revealed to act as a miRNA sponge for miR-933 and then regulated the expression of Smad2. CONCLUSIONS Results from the present study suggest that TFAP2A-AS1 acts as a tumor suppressor in BC via the miR-933/SMAD2 axis. |
| Databáze: | OpenAIRE |
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