Post-transfusion hepatitis C virus infection among β-thalassaemic individuals with associated clinical parameters
| Autor: | Aritra Biswas, Maitreyee Bhattacharyya, Prosanto Chowdhury, Kallol Saha, Provash Chandra Sadhukhan, Debyojyoti Bhattacharya, Rushna Firdaus |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Blood transfusion Adolescent Genotype Hepatitis C virus medicine.medical_treatment Iron 030106 microbiology Population India lcsh:Medicine Hepacivirus medicine.disease_cause Gastroenterology General Biochemistry Genetics and Molecular Biology Virus Hepatitis C virus - serum ferritin - transfusion transmitted infections - β-thalassaemia 03 medical and health sciences Young Adult Internal medicine medicine Humans Blood Transfusion transfusion transmitted infections education Child education.field_of_study business.industry beta-Thalassemia lcsh:R serum ferritin virus diseases Alanine Transaminase General Medicine β-thalassaemia Middle Aged Hepatitis C Reverse transcriptase digestive system diseases Child Preschool RNA Viral Original Article Liver function business Viral load |
| Zdroj: | Indian Journal of Medical Research, Vol 147, Iss 6, Pp 581-587 (2018) The Indian Journal of Medical Research |
| ISSN: | 0971-5916 |
| Popis: | Background & objectives: Multiple transfusions in β-thalassaemia patients undergoing regular transfusion regimen are at a risk of developing transfusion transmitted infections, including hepatitis C virus (HCV). The present study was conducted to investigate the association of HCV viraemia and genotype with clinical parameters in HCV seroreactive β-thalassaemic individuals. Methods: A total of 172 HCV seroreactive β-thalassaemic individuals aged between 2-35 yr with at least 25 units of blood transfusion were catagorized into four groups (2-12 yr, group 1; 13-19 yr, group 2; 20-29 yr, group 3; 30-35 yr, group 4). Aged matched control samples (n=87; β-thalassaemics without HCV infection) were also included. HCV RNA was detected by nested reverse transcriptase polymerase chain reaction (RT-PCR) based on 5’ UTR of HCV genome, viral load was determined by real-time RT-PCR. Nested RT-PCR amplified partial core region was used for DNA sequencing. Liver function parameters [serum total bilirubin, alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] were also determined. Results: Of the 172 HCV seroreactive individuals, 59.30 per cent (n=102) were HCV RNA positive. HCV viral load ranged from 173 to 32.04×10[5] IU/ml; 87.65 per cent were infected with HCV genotype 3. Liver enzymes, such as ALT, AST and serum total bilirubin were significantly elevated in all age groups compared to control groups. Serum ferritin levels were found to be high in all individuals, but 16.27 per cent of HCV-infected individuals with >10,000 IU/ml viral load also showed high ferritin levels (>1500 μg/l) where the majority of them were infected with HCV genotype 3. Interpretation & conclusions: HCV genotype 3 was the major circulating genotype among β-thalassaemia patients in this region. Our findings indicated an association between HCV replication and hepatic iron load and also highlighted the need for sensitive quantitative RT-PCR-based detection of HCV RNA in the high risk population. |
| Databáze: | OpenAIRE |
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