Site-Selective RNA Splicing Nanozyme: DNAzyme and RtcB Conjugates on a Gold Nanoparticle
| Autor: | Kevin Yehl, Roxanne Glazier, Jessica R. Petree, Brendan R Deal, Khalid Salaita, Kornelia Galior |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
RNA Splicing Metal Nanoparticles 02 engineering and technology RNA Biochemistry Biochemistry Article Amino Acyl-tRNA Synthetases 03 medical and health sciences RNA ligase biology Escherichia coli Proteins Ribozyme RNA General Medicine DNA Catalytic 021001 nanoscience & nanotechnology Molecular biology Cell biology Post-transcriptional modification 030104 developmental biology RNA editing Transfer RNA RNA splicing biology.protein Molecular Medicine Gold RNA Splice Sites 0210 nano-technology |
| Zdroj: | ACS chemical biology. 13(1) |
| ISSN: | 1554-8937 |
| Popis: | Modifying RNA through either splicing or editing is a fundamental biological process for creating protein diversity from the same genetic code. Developing novel chemical biology tools for RNA editing has potential to transiently edit genes and to provide a better understanding of RNA biochemistry. Current techniques used to modify RNA include the use of ribozymes, adenosine deaminase and tRNA endonucleases. Herein, we report a nanozyme that is capable of splicing of virtually any RNA stem-loop. This nanozyme is comprised of a gold nanoparticle functionalized with three enzymes: two catalytic DNA strands with ribonuclease function and an RNA ligase. The nanozyme cleaves and then ligates RNA targets, performing a splicing reaction that is akin to the function of the spliceosome. Our results show that the three-enzyme reaction can remove a 19 nt segment from a 67 nt RNA loop with up to 66% efficiency. The complete nanozyme can perform the same splice reaction at 10% efficiency. These splicing nanozymes represent a new promising approach for gene manipulation that has potential for applications in living cells. |
| Databáze: | OpenAIRE |
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