Modulation of ovine SBD-1 expression by Saccharomyces cerevisiae in ovine ruminal epithelial cells
| Autor: | Yun-he Wang, Xin Jin, Chenguang Du, Hua-er Bao, Yin-feng Yang, Yan-ru Fan, Siri-guleng Xu, Man Zhang, Xue-min Zhu, Qiao-zhen Tian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Rumen beta-Defensins p38 mitogen-activated protein kinases Enzyme-Linked Immunosorbent Assay Saccharomyces cerevisiae Real-Time Polymerase Chain Reaction SBD-1 03 medical and health sciences 0302 clinical medicine Immune system Downregulation and upregulation Gene expression Animals Secretion Signalling pathway Cells Cultured Modulation Innate immune system Sheep lcsh:Veterinary medicine General Veterinary Kinase Chemistry Ruminal epithelium General Medicine Cell biology TLR2 030104 developmental biology Gene Expression Regulation Gastric Mucosa lcsh:SF600-1100 Female 030215 immunology Research Article |
| Zdroj: | BMC Veterinary Research, Vol 14, Iss 1, Pp 1-10 (2018) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-018-1445-9 |
| Popis: | Background The ovine rumen is involved in host defense responses and acts as the immune interface with the environment. The ruminal mucosal epithelium plays an important role in innate immunity and secretes antimicrobial innate immune molecules that have bactericidal activity against a variety of pathogens. Defensins are cationic peptides that are produced by the mucosal epithelia and have broad-spectrum antimicrobial activity. Sheep β-defensin-1 (SBD-1) is one of the most important antibacterial peptides in the rumen. The expression of SBD-1 is regulated by the probiotic, Saccharomyces cerevisiae (S.c); however, the regulatory mechanism has not yet been elucidated. In the current study, the effects of S.c on the expression and secretion of SBD-1 in ovine ruminal epithelial cells were investigated using quantitative real-time PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA). In addition, specific inhibitors were used to block the nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB), p38, JNK, and ERK1/2 signalling pathways separately or simultaneously, to determine the regulatory mechanism(s) governing S.c-induced SBD-1 upregulation. Results Incubation with S.c induced release of SBD-1 by ovine ruminal epithelial cells, with SBD-1 expression peaking after 12 h of incubation. The highest SBD-1 expression levels were achieved after treatment with 5.2 × 107 CFU∙mL− 1 S.c. Treatment with S.c resulted in significantly increased NF-κB, p38, JNK, ERK1/2, TLR2, and MyD88 mRNA expression. Whereas inhibition of mitogen-activated protein kinases (MAPKs) and NF-κB gene expression led to a decrease in SBD-1 expression. Conclusions S.c was induced SBD-1 expression and the S.c-induced up-regulation of SBD-1 expression may be related to TLR2 and MyD88 in ovine ruminal epithelial cells. This is likely simultaneously regulated by the MAPKs and NF-κB pathways with the p38 axis of the MAPKs pathway acting as the primary regulator. Thus, the pathways regulating S.c-induced SBD-1 expression may be related to TLR2-MyD88-NF-κB/MAPKs, with the TLR2-MyD88-p38 component of the TLR2-MyD88-MAPKs signalling acting as the main pathway. |
| Databáze: | OpenAIRE |
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