Donor-derived CD4(+)/CCR7(+) T-cell partial selective depletion does not alter acquired anti-infective immunity
| Autor: | S Thiant, B. Choufi, Ibrahim Yakoub-Agha, Jacques Trauet, Myriam Labalette |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Receptors CCR7 Lymphocyte T cell Graft vs Host Disease Succinimides C-C chemokine receptor type 7 Enzyme-Linked Immunosorbent Assay Cell Separation Biology Tritium Lymphocyte Depletion Chemokine receptor Interferon-gamma Immune system Immunity medicine Humans Bone Marrow Transplantation Cell Proliferation Fluorescent Dyes Transplantation Hematology medicine.disease Flow Cytometry Fluoresceins Tissue Donors Graft-versus-host disease medicine.anatomical_structure Hematologic Neoplasms Lymphocyte Transfusion Immunology Acute Disease Female |
| Zdroj: | Bone marrow transplantation. 49(5) |
| ISSN: | 1476-5365 |
| Popis: | In previous studies, we observed that a high proportion of donor-derived CD4(+) T cells expressing the chemokine receptor 7 (CCR7) was a major determinant of acute GVHD, without interfering with the incidence of other post-transplant outcomes, especially relapse and nonrelapse mortality rates. Here, we investigated in vitro the impact of partially selective CD4(+)/CCR7(+) T lymphocytes on acquired anti-infective immune response in 10 donors who underwent G-CSF-primed PBSC collection. Similar quantitative and functional proliferative reactions were observed in lymphocyte cultures in the presence of adenovirus and pp65 Ags with unmanipulated and partially depleted donor samples. No responses were observed in the presence of human T-cell lymphotropic virus type 1 used as a negative control. These results complete the proof of concept needed to build a clinical trial investigating partially selective CD4(+)/CCR7(+) T cell-depleted allo-SCT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|