FecX Bar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep
| Autor: | Ahmed Rejeb, Stéphane Fabre, Florent Woloszyn, Narjess Lassoued, Zohra Benkhlil, Mourad Rekik, Sonia Bedhiaf-Romdhani, Mohamed Aouina |
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| Přispěvatelé: | INRA Tunisie, Lab Prod Anim & Fourrageres, Université de Carthage - University of Carthage, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Ecole Natl Med Vet, University of La Manouba, International Center for Agricultural Research in the Dry Areas (ICARDA), ANR 2010 BLAN 1608 01, École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université de la Manouba [Tunisie] (UMA), International Center for Agricultural Research in the Dry Areas [Syrie] (ICARDA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Barbarine Ovulation endocrine system lcsh:QH426-470 Sterility [SDV]Life Sciences [q-bio] Major gene Biology Growth differentiation factor-9 medicine.disease_cause Frameshift mutation 03 medical and health sciences BMP15 Genotype Genetics medicine Allele Genetics (clinical) Mutation Sheep Bone morphogenetic protein 15 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science lcsh:Genetics 030104 developmental biology barbarine bmp15 major gene ovulation prolificacy sheep Prolificacy Flock |
| Zdroj: | BMC Genetics, Vol 18, Iss 1, Pp 1-10 (2017) BMC Genetics BMC Genetics, BioMed Central, 2017, 18 (1), Non paginé. ⟨10.1186/s12863-017-0510-x⟩ BMC Genetics 1 (18), Non paginé. (2017) |
| ISSN: | 1471-2156 |
| DOI: | 10.1186/s12863-017-0510-x |
| Popis: | Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the “W” flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the “W” flock, and the entire coding sequences of GDF9 and BMP15 were sequenced. We evidenced a novel polymorphism in the exon 1 of the BMP15 gene associated with increased prolificacy and sterility. This novel mutation called FecX Bar is a composite polymorphism associating a single nucleotide substitution (c.301G > T), a 3 bp deletion (c.302_304delCTA) and a C insertion (c.310insC) in the ovine BMP15 cDNA leading to a frame shift at protein position 101. Calculated in the “W” flock, the FecX Bar allele increased OR by 0.7 ova and LS by 0.3 lambs (p = 0.08). As for already identified mutations, homozygous females carrying FecX Bar exhibited streaky ovaries with a blockade at the primary stage of folliculogenesis as shown by histochemistry. Our investigation demonstrates a new mutation in the BMP15 gene providing a valuable genetic tool to control fecundity in Tunisian Barbarine, usable for diffusion program into conventional flocks looking for prolificacy improvement. |
| Databáze: | OpenAIRE |
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