Nitric oxide from mononuclear cells may be involved in platelet responsiveness to aspirin

Autor:	Antonio Segura, José J. Zamorano-León, Carlos Macaya, Javier Modrego, Vicente Lahera, Pablo Rodríguez, Luis Azcona, Antonio J. López-Farré, Reddy Guerra
Rok vydání:	2014
Předmět:	Blood Platelets Male Nitric Oxide Synthase Type III Clinical Biochemistry Drug Resistance Nitric Oxide Synthase Type II Coronary Artery Disease Pharmacology Nitric Oxide Biochemistry Peripheral blood mononuclear cell Nitric oxide chemistry.chemical_compound Enos Humans Medicine Platelet Platelet activation Aged Aspirin biology Interleukin-6 business.industry Vitamin D-Binding Protein General Medicine biology.organism_classification digestive system diseases In vitro surgical procedures operative chemistry Immunology Leukocytes Mononuclear Platelet aggregation inhibitor Female business Platelet Aggregation Inhibitors
Zdroj:	European Journal of Clinical Investigation. 44:463-469
ISSN:	0014-2972
DOI:	10.1111/eci.12252
Popis:	Background Several mechanisms have been proposed to explain why some platelets have a reduced response to aspirin (ASA). Among them, it was reported an increased circulating level of vitamin-D-binding protein (DBP). In addition, nitric oxide (NO) released from mononuclear cells was involved in the antiplatelet effects of ASA. The aim was to analyse the relationship between platelet response to ASA and both NO generation and vitamin-D-binding protein content in mononuclear cells. Materials and methods Mononuclear cells were obtained from patients with stable coronary artery disease that were divided by a platelet functionality test (PFA-100) as ASA-sensitive (n = 23) and ASA resistant (n = 27). Results Both the release of NO (determined by nitrite + nitrate concentration) and the expression of endothelial-type NO synthase (eNOS) were higher in mononuclear cells from ASA sensitive as compared with those from ASA-resistant patients. There was a positive correlation between either the release of NO and the expression of eNOS protein in mononuclear cells with the ability of ASA to inhibit platelet activity. DBP content in mononuclear cells was higher in ASA resistant than in ASA sensitive. The level of DBP content in mononuclear cells was negatively associated with the ability of ASA to inhibit platelets. However, in vitro experiments suggested that there was no association between DBP and NO production by mononuclear cells. Conclusions Mononuclear cells from patients with platelets with lower responsiveness to ASA showed a reduced ability to produce NO.
Databáze:	OpenAIRE
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