Quantitative PCR Assays for Detection of Five Arctic Fish Species: Lota lota, Cottus cognatus, Salvelinus alpinus, Salvelinus malma, and Thymallus arcticus from Environmental DNA
| Autor: | John R. Olson, Karen E. Mock, Torrey W. Rodgers, Stephen L. Klobucar |
|---|---|
| Přispěvatelé: | Springer Netherlands |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Cottus cognatus Zoology Grayling biology.organism_classification 010603 evolutionary biology 01 natural sciences Alaska North Slope Fishery 03 medical and health sciences 030104 developmental biology Arctic Arctic char Other Life Sciences Genetics Species detection Sculpin Environmental DNA eDNA Ecology Evolution Behavior and Systematics Thymallus arcticus geographic locations Salvelinus |
| Zdroj: | Ecology Center Publications |
| Popis: | The North Slope of Alaska contains arctic fish populations that are important for subsistence of local human populations, and are under threat from natural resource extraction and climate change. We designed and evaluated four quantitative PCR assays for the detection of environmental DNA from five Alaskan fish species present on the North Slope of Alaska: burbot (Lota lota), arctic char (Salvelinus alpinus), Dolly Varden (Salvelinus malma), arctic grayling (Thymallus arcticus), and slimy sculpin (Cottus cognatus). All assays were designed and tested for species specificity and sensitivity, and all assays detected target species from filtered water samples collected from the field. These assays will enable efficient and economical detection and monitoring of these species in lakes and rivers. This in turn will provide managers with improved knowledge of current distributions and future range shifts associated with climate and development threats, enabling more timely management. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|