Phytochemical Analysis and Antinociceptive Properties of Hydroalcoholic Extracts of Aleurites moluccanus Bark
| Autor: | Luiz G. R. D. Chagas, Simone Reichert, Christiane Meyre-Silva, Valdir Cechinel-Filho, Ana Elisa Gonçalves, Ivania T. A. Schuquel, Marcelo Tomczak, Márcia Maria de Souza |
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| Rok vydání: | 2021 |
| Předmět: |
Aleurites
Phytochemicals Pharmaceutical Science Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Drug Discovery medicine Plant Bark Animals Edema Atraric acid 030304 developmental biology Pharmacology 0303 health sciences Analgesics Ethanol biology Traditional medicine Plant Extracts Organic Chemistry biology.organism_classification Carrageenan Complementary and alternative medicine chemistry Phytochemical visual_art Hyperalgesia visual_art.visual_art_medium Molecular Medicine Bark medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Planta medica. 87(10-11) |
| ISSN: | 1439-0221 |
| Popis: | Aleurites moluccanus is used in folk medicine to treat many diseases including pain and inflammatory processes in general. Considering the potential of the leaf extract, evidenced in a previous study, the present study investigates the antinociceptive and anti-inflammatory properties of the hydroethanolic extract of A. moluccanus bark and isolated compounds in animal models of pain. The antinociceptive and anti-inflammatory activities of A. moluccanus bark were evaluated through hyperalgesia induced by carrageenan, PGE2, cytokines, bradykinin, epinephrine, Freundʼs complete adjuvant, and lipopolysaccharide. Five compounds were isolated from the dichloromethane bark extract: acetyl aleuritolic acid, atraric acid, spruceanol, (5β,10α)-12-hydroxy-13-methoxy-8,11,13-podocarpatrien-3-one and sonderianol. To optimize the extraction conditions, ethanol 50, 70, and 90°GL were used as extracting solvent, in a 1 : 20 (w/v) drug : solvent ratio, under stirring at room temperature for 4 h. The extracts were named AMC50, AMC70, and AMC90, respectively. These extracts were administered to mice (250 mg/kg, p. o.) with reduced mechanical hyperalgesia activity in the carrageenan test. Of these, AMC90 showed the best results. Pure (5β,10α)-12-hydroxy-13-methoxy-8,11,13-podocarpatrien-3-one showed a beneficial effect for up to 48 hours after the administration of carrageenan, while acetyl aleuritolic acid was effective only in the first hour. AMC90 was able to reverse the analgesia induced only by prostaglandin E2 and tumor necrosis factor. We also induced hyperalgesia using the lipopolysaccharide and Freundʼs complete adjuvant models, with positive results. These results support the antinociceptive and anti-inflammatory activity of A. moluccanus bark extract. The observed effects are partly due to the presence of acetyl aleuritolic acid, atraric acid, and (5β,10α)-12-hydroxy-13-methoxy-8,11,13-podocarpatrien-3-one. |
| Databáze: | OpenAIRE |
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