Mass Spectrometry of Oligonucleotides
| Autor: | Selina T. M. Monn, Jan M. Tromp, Stefan Schürch |
|---|---|
| Jazyk: | němčina |
| Rok vydání: | 2005 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Bioanalysis Electrospray Collision-induced dissociation Protein mass spectrometry Sequence analysis Tandem mass spectrometry Electrospray ionization Oligonucleotides Top-down proteomics Mass spectrometry Biochemistry Dissociation (chemistry) chemistry.chemical_compound Genetics QD1-999 Metal-oligonucleotide complexes Chromatography Base Sequence Sequence Analysis RNA Chemistry Oligonucleotide DNA Sequence Analysis DNA General Chemistry General Medicine Combinatorial chemistry Molecular Medicine Rna Iron(iii) |
| Zdroj: | CHIMIA, Vol 59, Iss 11 (2005) |
| ISSN: | 2673-2424 0009-4293 |
| Popis: | Expanding research in the field of modified oligonucleotides demands suitable analytical tools for size and purity verification of known compounds and accurate structure elucidation of unknowns. There is a need for characterization of the types and sites of modifications in oligonucleotides and to identify and sequence selected candidates originating from synthesis. The potential of electrospray tandem mass spectrometry (ESI-MS/MS) for structural characterization and sequencing of oligonucleotides is demonstrated. The fundamental behavior of DNA, RNA, and selected modified oligonucleotides in gas-phase is shown. Since gas-phase dissociation does not demand specific structural prerequisites, the method bears a great potential for rapid and most accurate characterization of modified oligonucleotides, e.g. from combinatorial libraries. |
| Databáze: | OpenAIRE |
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