A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2′-deoxyguanosine measurements in urine and other biologic matrices: a one-year evaluation of methods
| Autor: | Mikhail B. Bogdanov, D R McCabe, Wayne R. Matson, M F Beal, R M Griffin |
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| Rok vydání: | 1999 |
| Předmět: |
DNA damage
Context (language use) Urine Sensitivity and Specificity Biochemistry chemistry.chemical_compound Physiology (medical) Electrochemistry Animals Humans Deoxyguanosine Animal body Cerebrospinal Fluid Chromatography Dialysis fluid Cerebral Palsy Amyotrophic Lateral Sclerosis Reproducibility of Results 8-Hydroxy-2'-deoxyguanosine Parkinson Disease DNA Reference Standards Oxidative Stress chemistry 8-Hydroxy-2'-Deoxyguanosine External blind Biomarkers Chromatography Liquid DNA Damage |
| Zdroj: | Free Radical Biology and Medicine. 27:647-666 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(99)00113-6 |
| Popis: | 8-Hydroxy-2'-deoxyguanosine (8OH2'dG) is a principal stable marker of hydroxyl radical damage to DNA. It has been related to a wide variety of disorders and environmental insults, and has been proposed as a useful systematic marker of oxidative stress. Analytic procedures for 8OH2'dG in DNA digests are well established; however, routine measurement of free 8OH2'dG in other body fluids such as urine or plasma has been problematic. This has hindered its evaluation as a general clinical, therapeutic monitoring, or environmental assessment tool. Therefore, we developed a liquid chromatography electrochemical column-switching system based on the use of the unique purine selectivity of porous carbon columns that allows routine accurate measurement of 8OH2'dG in a variety of biologic matrices. This paper describes the rationale of the system design and the protocols developed for 8OH2'dG in urine, plasma, cerebrospinal fluid, tissue, DNA, saliva, sweat, kidney dialysis fluid, foods, feces, culture matrix, and microdialysates. Concentrations in both human and animal body fluids and tissues are reported. The system performance is discussed in the context of a 1-year evaluation of the methods applied to approximately 3600 samples, using internal quality control and external blind testing to determine long-term accuracy. The methods are reliable and accurate, and therefore should prove useful in assessing the role and utility of oxidative DNA damage in aging and human illness. |
| Databáze: | OpenAIRE |
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