Rat kidney MAP17 induces cotransport of Na-mannose and Na-glucose inXenopus laevisoocytes
| Autor: | J. J. Aramayona, Victor Sorribas, Manuel Sarasa, Tatiana Blasco, Ana I. Alcalde, Julia Catalán |
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| Rok vydání: | 2003 |
| Předmět: |
Monosaccharide Transport Proteins
Physiology Molecular Sequence Data Xenopus Golgi Apparatus Mannose Rat kidney Biology Kidney Protein Structure Secondary Cell Line Xenopus laevis chemistry.chemical_compound Animals Tissue Distribution Amino Acid Sequence Base Sequence Microvilli Uphill Transport Sodium Membrane Proteins Renal Reabsorption Biological Transport Opossums biology.organism_classification Neoplasm Proteins Rats Biochemistry chemistry Oocytes Female Cotransporter Homeostasis |
| Zdroj: | American Journal of Physiology-Renal Physiology. 285:F799-F810 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00149.2003 |
| Popis: | Renal reabsorption is the main mechanism that controls mannose homeostasis. This takes place through a specific Na-coupled uphill transport system, the molecular identity of which is unknown. We prepared and screened a size-selected rat kidney cortex cDNA library through the expression of mannose transport in Xenopus laevis oocytes. We have identified a membrane protein that induces high-affinity and specific Na-dependent transport of d-mannose and d-glucose in X. laevis oocytes, most likely through stimulation of the capacity of an endogenous transport system of the oocyte. Sequencing has revealed that the cDNA encodes the counterpart of the human membrane-associated protein MAP17, previously known by its overexpression in renal, colon, lung, and breast carcinomas. We show that MAP17 is a 12.2-kDa nonglycosylated membrane protein that locates to the brush-border plasma membrane and the Golgi apparatus of transfected cells and that it is expressed in the proximal tubules of the kidney cortex and in the spermatids of the seminiferous tubules. It spans twice the cell membrane, with both termini inside the cell, and seems to form homodimers through intracellular Cys55, a residue also involved in transport expression. MAP17 is responsible for mannose transport expression in oocytes by rat kidney cortex mRNA. The induced transport has the functional characteristics of a Na-glucose cotransporter (SGLT), because d-glucose and α-methyl-d-glucopyranoside are also accepted substrates that are inhibited by phloridzin. The corresponding transporter from the proximal tubule remains to be identified, but it is different from the known mammalian SGLT-1, -2, and -3. |
| Databáze: | OpenAIRE |
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