Differential capacity of CD90+ cells in autophagy activation following chemotherapy in hepatocellular carcinoma
| Autor: | Caecilia Hc Sukowati, Deborah Bonazza, Claudio Tiribelli, Cristina Bottin, Long Dc Tran, Gianluca Tell, Hoa Lt Pham, Huy Q. Do, Thao P. T. Doan, An B Luong, Nhung Truong |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
cancer stem cells autophagy Carcinoma Hepatocellular Cell Culture Techniques Specialties of internal medicine 03 medical and health sciences 0302 clinical medicine Cancer stem cell Cell Line Tumor Medicine Humans MTT assay Doxorubicin CD90 Antibiotics Antineoplastic Hepatology business.industry Autophagy Liver Neoplasms Cancer chemoresistance General Medicine hepatocellular carcinoma Middle Aged medicine.disease RC581-951 030220 oncology & carcinogenesis Hepatocellular carcinoma embryonic structures Cancer research Thy-1 Antigens 030211 gastroenterology & hepatology Cancer biomarkers Female business Microtubule-Associated Proteins medicine.drug |
| Zdroj: | Annals of Hepatology, Vol 19, Iss 6, Pp 645-652 (2020) |
| Popis: | Introduction and Objectives. Analysis of cancer biomarkers is an important tool in developing targeted-therapy and in modulating chemoresistance. Here, we analyze the relevance of CD90, a marker of cancer stem cells (CSC) in hepatocellular carcinoma (HCC) and its correlation with autophagy. Materials and Methods. For in vivo study, 86 specimens were collected from 43 patients undergoing liver resections. In each patient, HCC nodule (HCC) and surrounding non-tumor (SNT) were collected. For in vitro study, HCC cells JHH6 subpopulations expressing CD90+ and CD90- were isolated using magnetic-sorter and confirmed by flow-cytometry. Upon doxorubicin treatment, autophagy turn-over was analyzed by RTqPCR for mRNA expression, Western blot for protein expression, and autophagosome staining for autophagy-flux. Cytotoxicity test was performed by MTT assay. Gene and protein analysis were performed in clinical samples together with immunohistostaining. Results. CD90 mRNA expression was higher in HCC than in SNT for 8-fold (p |
| Databáze: | OpenAIRE |
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