Gibberellin Oxidase Gene Family in L. chinense: Genome-Wide Identification and Gene Expression Analysis

Autor: Ye Lu, Haoxian Qu, Cheng Tielong, Jisen Shi, Jinhui Chen, Pengkai Wang, Zhaodong Hao, Zijian Cao, Hu Lingfeng, Guoxia Xue
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
abiotic stress
Liriodendron
QH301-705.5
phytohormone
medicine.disease_cause
01 natural sciences
Article
Catalysis
Inorganic Chemistry
gibberellin oxidase
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Stress
Physiological
Arabidopsis
Gene expression
medicine
Gene family
Biology (General)
Physical and Theoretical Chemistry
Codon Usage
Promoter Regions
Genetic
QD1-999
Molecular Biology
Abscisic acid
Gene
Spectroscopy
Genetics
Mutation
biology
Organic Chemistry
food and beverages
General Medicine
biology.organism_classification
Gibberellins
Computer Science Applications
Chemistry
030104 developmental biology
chemistry
Multigene Family
Codon usage bias
gene family
L. chinense
Gibberellin
Oxidoreductases
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7167, p 7167 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 13
ISSN: 1422-0067
Popis: GAox is a key enzyme for the transformation of gibberellins, and belongs to the 2-ketoglutarate dependent dioxygenase gene family (2ODD). However, a systematic analysis of GAox in the angiosperm L. chinense has not yet been reported. Here, we identified all LcGAox gene family members in L. chinense, which were classified into the three subgroups of GA20ox, C19GA2ox, and C20GA2ox. Comparison of the gene structure, conserve motifs, phylogenetic relationships, and syntenic relationships of gibberellin oxidase gene families in different species indicated that the gene functional differences may be due to the partial deletion of their domains during evolution. Furthermore, evidence for purifying selection was detected between orthologous GAox genes in rice, grape, Arabidopsis, and L. chinense. Analysis of the codon usage patterns showed that mutation pressure and natural selection might have induced codon usage bias in angiosperms
however, the LcGAox genes in mosses, lycophytes, and ambarella plants exhibited no obvious codon usage preference. These results suggested that the gibberellin oxidase genes were more primitive. The gene expression pattern was analyzed in different organs subjected to multiple abiotic stresses, including GA, abscisic acid (ABA), and chlormequat (CCC) treatment, by RNA-seq and qRT-PCR, and the stress- and phytohormone-responsive cis-elements were counted. The results showed that the synthesis and decomposition of GA were regulated by different LcGAox genes in the vegetative and reproductive organs of L. chinense, and only LcGA2ox1,4, and 7 responded to the NaCl, polyethylene glycol, 4 °C, GA, ABA, and CCC treatment in the roots, stems, and leaves of seedlings at different time periods, revealing the potential role of LcGAox in stress resistance.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje