Simultaneous Quantification of Vesicle Size and Catecholamine Content by Resistive Pulses in Nanopores and Vesicle Impact Electrochemical Cytometry
| Autor: | Xin-Wei Zhang, Amir Hatamie, Andrew G. Ewing |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Nanoparticle
010402 general chemistry 01 natural sciences Biochemistry Catalysis Exocytosis Nanopores Colloid and Surface Chemistry Catecholamines Animals Chromaffin Granules Particle Size Electrodes Chemistry Vesicle Communication Pipette Intracellular vesicle General Chemistry Electrochemical Techniques Microvesicles 0104 chemical sciences Nanopore Biophysics Cattle Cytometry |
| Zdroj: | Journal of the American Chemical Society |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | We have developed the means to simultaneously measure the physical size and count catecholamine molecules in individual nanometer transmitter vesicles. This is done by combining resistive pulse (RP) measurements in a nanopore pipette and vesicle impact electrochemical cytometry (VIEC) at an electrode as the vesicle exits the nanopore. Analysis of freshly isolated bovine adrenal vesicles shows that the size and internal catecholamine concentration of vesicles varies with the occurrence of a dense core inside the vesicles. These results might benefit the understanding about the vesicles maturation, especially involving the “sorting by retention” process and concentration increase of intravesicular catecholamine. The methodology is applicable to understanding soft nanoparticle collisions on electrodes, vesicles in exocytosis and phagocytosis, intracellular vesicle transport, and analysis of electroactive drugs in exosomes. |
| Databáze: | OpenAIRE |
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