The Sustainable Release of Vancomycin and Its Degradation Products From Nanostructured Collagen/Hydroxyapatite Composite Layers
| Autor: | Lukáš Horný, Monika Šupová, Šárka Rýglová, Zbyněk Sucharda, M. Braun, Tomáš Suchý, Eva Klapkova, Rastislav Ballay, Hynek Chlup, Margit Žaloudková, Jan Veselý, František Denk |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Hydrochloride
Vancomycin Hydrochloride Pharmaceutical Science 02 engineering and technology 010402 general chemistry 01 natural sciences High-performance liquid chromatography Methylamines Minimum inhibitory concentration chemistry.chemical_compound Drug Delivery Systems Vancomycin medicine Drug Carriers Chromatography Chemistry Elution 021001 nanoscience & nanotechnology Controlled release Nanostructures 0104 chemical sciences Carbodiimides Durapatite Delayed-Action Preparations Genipin Nanoparticles Collagen 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Pharmaceutical Sciences. 105:1288-1294 |
| ISSN: | 0022-3549 |
| Popis: | Infections of the musculoskeletal system present a serious problem with regard to the field of orthopedic and trauma medicine. The aim of the experiment described in this study was to develop a resorbable nanostructured composite layer with the controlled elution of antibiotics. The layer is composed of collagen, hydroxyapatite nanoparticles, and vancomycin hydrochloride (10 wt%). The stability of the collagen was enhanced by means of cross-linking. Four cross-linking agents were studied, namely an ethanol solution, a phosphate buffer solution of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide, genipin, and nordihydroguaiaretic acid. High performance liquid chromatography was used so as to characterize the in vitro release rates of the vancomycin and its crystalline degradation antibiotically inactive products over a 21-day period. The maximum concentration of the released active form of vancomycin (approximately 265 mg/L) exceeded the minimum inhibitory concentration up to an order of 17 times without triggering the burst releasing effect. At the end of the experiment, the minimum inhibitory concentration was exceeded by up to 6 times (approximately 100 mg/L). It was determined that the modification of collagen with hydroxyapatite nanoparticles does not negatively influence the sustainable release of vancomycin. The balance of vancomycin and its degradation products was observed after 14 days of incubation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|