High-resolution macromolecular structure determination using CCD detectors and synchrotron radiation
| Autor: | Eric F. Eikenberry, Richard L. Walter, Sandor L. Barna, Mark W. Tate, Daniel J. Thiel, Michael E. Wall, Steven E. Ealick, Sol M. Gruner |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Diffraction
X-ray detector Physics::Instrumentation and Detectors Lipoxygenase High resolution Synchrotron radiation Crystallography X-Ray law.invention Optics Cellulase law Structural Biology Amino Acid Sequence Pentosyltransferases Molecular Biology Physics Binding Sites Fourier Analysis business.industry synchrotron radiation Detector Proteins macromolecular structure Synchrotron High-energy X-rays Viruses Soybeans Ccd detector business charge-coupled device (CCD) Synchrotrons |
| Zdroj: | Structure (London, England : 1993). 3(8) |
| ISSN: | 0969-2126 |
| Popis: | Background: Synchrotron radiation sources have made impressive contributions to macromolecular crystallography. The delay in development of appropriate X-ray detectors has, however, been a significant limitation to their efficient use. New technologies, based on charge-coupled devices (CCDs), provide capabilities for faster, more accurate, automated data collection. Results A CCD-based X-ray detector has been developed for use in macromolecular crystallography and has been in operation for about one and a half years at the Cornell High Energy Synchrotron Source. It has been used for a variety of crystallographic projects, including a number of high-resolution structural studies. The statistical quality of the data, the detector's ease and efficiency of use, and the growing number of structural results illustrate the practical utility of this new detector system. Conclusion The new detector has enhanced capabilities for measuring diffraction patterns from crystals of macromolecules, especially at high resolution, when the X-ray intensities are weak. The survey of results described here ranges from virus crystallography to weakly diffracting small-molecule structure determination and demonstrates the potential of CCD detectors when combined with synchrotron radiation sources. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|