Ectopic Neo-Formed Intracellular Membranes in Escherichia coli: A Response to Membrane Protein-Induced Stress Involving Membrane Curvature and Domains
| Autor: | Stéphane Orlowski, Nadège Jamin, Christine Jaxel, Manuel Garrigos, Annie Frelet-Barrand |
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| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Protéines et Systèmes Membranaires (LPSM), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), ANR-17-CE11-0015,CAVEOTANK,Production de protéines membranaires recombinantes insérées dans les membranes de cavéoles hétérologues(2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QR1-502 Review Biochemistry lcsh:Microbiology [SPI.MAT]Engineering Sciences [physics]/Materials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Protein structure Membrane Microdomains Stress Physiological Cardiolipin Escherichia coli [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics Molecular Biology ComputingMilieux_MISCELLANEOUS [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] vesicles Vesicle Membrane Proteins intracellular membranes membrane protein overexpression [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] 030104 developmental biology Membrane Membrane protein chemistry membrane domains Membrane curvature Cytoplasm membrane curvature Biophysics tubules 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Biomolecules Biomolecules, MDPI, 2018, 8 (3), pp.88. ⟨10.3390/biom8030088⟩ Biomolecules, Vol 8, Iss 3, p 88 (2018) Biomolecules, 2018, 8 (3), pp.88. ⟨10.3390/biom8030088⟩ |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom8030088⟩ |
| Popis: | International audience; Bacterial cytoplasmic membrane stress induced by the overexpression of membrane proteins at high levels can lead to formation of ectopic intracellular membranes. In this review, we report the various observations of such membranes in Escherichia coli, compare their morphological and biochemical characterizations, and we analyze the underlying molecular processes leading to their formation. Actually, these membranes display either vesicular or tubular structures, are separated or connected to the cytoplasmic membrane, present mono- or polydispersed sizes and shapes, and possess ordered or disordered arrangements. Moreover, their composition differs from that of the cytoplasmic membrane, with high amounts of the overexpressed membrane protein and altered lipid-to-protein ratio and cardiolipin content. These data reveal the importance of membrane domains, based on local specific lipid–protein and protein–protein interactions, with both being crucial for local membrane curvature generation, and they highlight the strong influence of protein structure. Indeed, whether the cylindrically or spherically curvature-active proteins are actively curvogenic or passively curvophilic, the underlying molecular scenarios are different and can be correlated with the morphological features of the neo-formed internal membranes. Delineating these molecular mechanisms is highly desirable for a better understanding of protein–lipid interactions within membrane domains, and for optimization of high-level membrane protein production in E. coli. |
| Databáze: | OpenAIRE |
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