Polystyrene nanoplastics and microplastics can act as Trojan horse carriers of benzo(a)pyrene to mussel hemocytes in vitro
| Autor: | Marta Barros, Alberto Katsumiti, Miren P. Cajaraville, María Paula Losada-Carrillo |
|---|---|
| Přispěvatelé: | European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microplastics
animal structures Hemocytes Chemical Phenomena Cell Survival Science Phagocytosis Membrane trafficking Biologics Cellular imaging medicine.disease_cause Article chemistry.chemical_compound Environmental biotechnology medicine polycyclic compounds Benzo(a)pyrene Animals Viability assay Particle Size Mytilus Animal biotechnology Multidisciplinary Assay systems Mussel Environmental sciences Ocean sciences chemistry Biochemistry Toxicity Medicine Pyrene Nanoparticles Polystyrenes Reactive Oxygen Species Genotoxicity Water Pollutants Chemical |
| Zdroj: | Scientific Reports Addi. Archivo Digital para la Docencia y la Investigación instname Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) |
| Popis: | [EN]In this work we studied the ability of polystyrene (PS) nanoplastics (NPs) and microplastics (MPs) to transfer benzo(a)pyrene (BaP) to mussel hemocytes and to produce toxic effects in vitro. For this, intracellular fate and toxicity of PS NPs (0.05 μm) and MPs (0.5 and 4.5 μm) alone or with BaP and of BaP alone were assessed. Particles of 0.05 and 0.5 µm largely aggregated in the exposure medium whereas presence of BaP reduced particle aggregation. Cells internalized PS NPs and MPs alone or with BaP and these were found inside and outside lysosomes, depending on their size. PS particles alone or with BaP were cytotoxic to hemocytes only at the highest concentrations tested. The same was true for most sublethal endpoints except for increased phagocytic activity provoked by NPs and 0.5 μm MPs at lower concentrations. Plastic particles appeared to be the main drivers for reduced plasma membrane integrity and increased phagocytic and lysosomal activities whereas BaP appeared to contribute more to reduced cell viability and phagocytosis and increased ROS production and genotoxicity. Overall, PS NPs and MPs can act as carriers of BaP to mussel hemocytes, rising concerns about risks plastics associated to pollutants may pose to aquatic organisms. This work was carried out within the EU Project PLASTOX (JPI Oceans 005/2015) and was funded by the Spanish MINECO (NACE Project, CTM2016-81130-R) and Basque Government (Consolidated Research Groups Grant IT1302-19). |
| Databáze: | OpenAIRE |
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