A new LC–MS/MS bioanalytical method for atenolol in human plasma and milk
| Autor: | Ei Mon Phyo Lwin, Cobus Gerber, Yunmei Song, Catherine Leggett, Sanjay Garg, Usha Ritchie, Sean Turner |
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| Přispěvatelé: | Lwin, Ei Mon Phyo, Gerber, Cobus, Song, Yunmei, Leggett, Catherine, Ritchie, Usha, Turner, Sean, Garg, Sanjay |
| Rok vydání: | 2017 |
| Předmět: |
Analyte
Bioanalysis Clinical Biochemistry Phenazone 01 natural sciences Analytical Chemistry Plasma 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Lactation Lc ms ms medicine Animals Humans Protein precipitation 030212 general & internal medicine General Pharmacology Toxicology and Pharmaceutics Chromatography human plasma Chemistry 010401 analytical chemistry human milk Reproducibility of Results General Medicine Reference Standards Atenolol atenolol 0104 chemical sciences Medical Laboratory Technology Milk medicine.anatomical_structure Human plasma Calibration Cattle Chromatography Liquid medicine.drug |
| Zdroj: | Bioanalysis. 9:517-530 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio-2016-0304 |
| Popis: | Aim: A new sensitive LC–MS/MS method for the quantification of atenolol in human plasma and milk has been developed for clinical lactation studies. Methods & results: Atenolol and the internal standard, phenazone, were extracted from biological matrices by protein precipitation. A Phenomenex®C-18 column and gradient chromatographic conditions were used for separation of the analyte, followed by detection with MS. Stability of samples was confirmed for atenolol in human plasma and milk for up to 3 months. Linearity range of 1–800 ng/ml (r2= 0.9995), the precision within 15% CV and the recovery of the analyte (80–100% range) were achieved. Conclusion: A new validated analytical method for atenolol in plasma and milk was developed. |
| Databáze: | OpenAIRE |
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