Overexpression of the peroxisome proliferator activated receptor α or the human c-Ha-ras transgene is not involved in tumorigenesis induced by di(2-ethylhexyl)phthalate in rasH2 mice
| Autor: | Kaoru Toyosawa, Nobuo Matsuoka, Kohji Tanaka, Yasuhiro Teranishi, Kenji Okugawa |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Genetically modified mouse endocrine system Cancer Research Transgene Receptors Cytoplasmic and Nuclear Peroxisome proliferator-activated receptor Mice Transgenic Biology medicine.disease_cause Adenoma Liver Cell Mice chemistry.chemical_compound Diethylhexyl Phthalate medicine Animals Humans RNA Messenger Transcription factor chemistry.chemical_classification Cell growth Phthalate Molecular biology Gene Expression Regulation Neoplastic Genes ras Oncology chemistry Peroxisome proliferator-activated receptor alpha Carcinogenesis Cell Division Transcription Factors |
| Zdroj: | Cancer Letters. 192:199-203 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/s0304-3835(02)00714-0 |
| Popis: | The mRNA profiles for peroxisome proliferator activated receptor (PPAR) alpha and human c-Ha-ras genes were determined by real-time semi-quantitative polymerase chain reaction analysis of hepatocellular adenomas induced by di(2-ethylhexyl)phthalate (DEHP) in transgenic mice carrying a human prototype c-Ha-ras gene (rasH2 mice). The mRNA levels were essentially equal in hepatocellular adenomas and adjacent non-neoplastic hepatocytes, in spite of a remarkable elevation in the cell proliferation index in tumors determined by anti-Ki-67 immunohistochemistry. From the results, it is concluded that overexpression of PPARalpha or the transgene is not associated with the liver tumorigenesis induced by DEHP in rasH2 mice. |
| Databáze: | OpenAIRE |
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