Liquid chromatographic determination of hyoscine (scopolamine) in urine using solid phase extraction
| Autor: | Roger F. Metcalfe, Peter R. Hurst, Robin Whelpton, Sally A. Saunders |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Metabolite Clinical Biochemistry Scopolamine Urine Biochemistry High-performance liquid chromatography Analytical Chemistry Coulometry Excretion chemistry.chemical_compound Reference Values Drug Discovery medicine Electrochemistry Humans Solid phase extraction Molecular Biology Chromatography High Pressure Liquid Pharmacology Detection limit Chromatography Chemistry Hydrolysis General Medicine Chromatography Thin Layer medicine.drug |
| Zdroj: | Biomedical chromatography : BMC. 6(4) |
| ISSN: | 0269-3879 |
| Popis: | A sensitive method for the determination of hyoscine (scopolamine) in urine is described. After concentration and "clean-up" on C18 and CN solid phase extraction columns, hyoscine was quantified by high performance liquid chromatography with coulometric detection (oxidation at +0.9 V). The limit of detection was 5 ng per sample and the precision for 5 mL samples containing 2 ng/mL was 12.3%. The method was applied to urine samples collected from 12 volunteers wearing Scopoderm TTS patches. The mean excretion rate of unmetabolized hyoscine was 0.45 micrograms/h and 87% of the total hyoscine was present as conjugates. Apohyoscine (aposcopolamine) was identified as a urinary metabolite. The significance of this with regard to hyoscine assays is discussed. |
| Databáze: | OpenAIRE |
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