A role for cell sex in stem cell-mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency

Autor: Johnny Huard, Jonathan B. Pollett, Robert T. Rubin, Rebecca C. Schugar, Aiping Lu, Jessica C. Tebbets, Joseph M. Feduska, Burhan Gharaibeh, Baohong Cao, Bridget M. Deasy, Bin Sun, Kenneth L. Urish
Rok vydání: 2007
Předmět:
Male
Cellular differentiation
1.1 Normal biological development and functioning
Cell
Biology
Regenerative Medicine
Medical and Health Sciences
Article
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Sex Factors
Underpinning research
medicine
Animals
Regeneration
2.1 Biological and endogenous factors
Aetiology
Muscle
Skeletal
Research Articles
030304 developmental biology
Oligonucleotide Array Sequence Analysis
0303 health sciences
Regeneration (biology)
Stem Cells
Gene Expression Profiling
Inbred mdx
Skeletal muscle
Cell Differentiation
Cell Biology
Skeletal
Biological Sciences
Stem Cell Research
Cell biology
Transplantation
medicine.anatomical_structure
Musculoskeletal
Immunology
Mice
Inbred mdx
Myeloid-derived Suppressor Cell
Muscle
Female
Stem cell
030217 neurology & neurosurgery
Stem Cell Transplantation
Developmental Biology
Zdroj: The Journal of cell biology, vol 177, iss 1
The Journal of Cell Biology
Popis: We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability in regeneration abilities. We show here that cell sex is a variable that considerably influences MDSCs' regeneration abilities. We found that the female MDSCs (F-MDSCs) regenerated skeletal muscle more efficiently. Despite using additional isolation techniques and cell cloning, we could not obtain a male subfraction with a regeneration capacity similar to that of their female counterparts. Rather than being directly hormonal or caused by host immune response, this difference in MDSCs' regeneration potential may arise from innate sex-related differences in the cells' stress responses. In comparison with F-MDSCs, male MDSCs have increased differentiation after exposure to oxidative stress induced by hydrogen peroxide, which may lead to in vivo donor cell depletion, and a proliferative advantage for F-MDSCs that eventually increases muscle regeneration. These findings should persuade researchers to report cell sex, which is a largely unexplored variable, and consider the implications of relying on cells of one sex.
Databáze: OpenAIRE
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