Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells
| Autor: | Miyuki Shimane, Wataru Nishida, Kenji Sobue, Shoji Miyata, Shin-Ichi Funahashi, Yuji Matsuzawa, Yoshihiro Chimori, Kazuhiro Kimura, Ken-ichiro Hayashi |
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| Jazyk: | angličtina |
| Předmět: |
Biophysics
Fluorescent Antibody Technique Chick Embryo Biology Biochemistry mRNA subtraction Smooth muscle Structural Biology In vivo Genetics medicine Animals RNA Messenger Molecular Biology Cadherin 6B Cells Cultured In Situ Hybridization Messenger RNA Kidney Retina Cadherin Cell adhesion molecule Embryogenesis Gene Expression Regulation Developmental Cell Differentiation Muscle Smooth Cell Biology Phenotypic modulation Oligonucleotides Antisense Cadherins musculoskeletal system Phenotype Cell biology Viscera medicine.anatomical_structure cardiovascular system tissues Biomarkers |
| Zdroj: | FEBS Letters. (1):67-71 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(00)01220-5 |
| Popis: | We used mRNA subtraction of differentiated and dedifferentiated smooth muscle cells (SMCs) to reveal the molecular mechanisms underlying the phenotypic modulation of SMCs. With this approach, we found that a 10 kb mRNA encoding a homotypic cell adhesion molecule, cadherin 6B, was strongly expressed in differentiated vascular and visceral SMCs, but not in the dedifferentiated SMCs derived from them. In vivo, cadherin 6B was expressed in vascular and visceral SMCs, in addition to brain, spinal cord, retina and kidney, at a late stage of chicken embryonic development. These results suggest that cadherin 6B is a novel molecular marker for vascular and visceral SMC phenotypes and is involved in the late differentiation of SMCs. |
| Databáze: | OpenAIRE |
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