Reversed-phase high-performance liquid chromatography of ketorolac and its application to bioequivalence studies in human serum
| Autor: | S. Khanna, S.S. Gangwal, K.C. Jindal, R.S. Chaudhary |
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| Rok vydání: | 1993 |
| Předmět: |
Analgesics
Chromatography Chemistry Coefficient of variation Anti-Inflammatory Agents Non-Steroidal Reproducibility of Results General Chemistry Reversed-phase chromatography Bioequivalence High-performance liquid chromatography Biological fluid body regions Ketorolac Pharmacokinetics Phase (matter) medicine Humans Tolmetin Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 614:180-184 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(93)80240-5 |
| Popis: | A reversed-phase high-performance liquid chromatographic assay was used to study the bioequivalence of the anti-inflammatory drug (+/-)-ketorolac in human volunteers. Following deproteinization of human serum with 5% zinc sulphate solution, ketorolac was chromatographed on a 10-microns octadecylsilica column using acetonitrile-water as mobile phase and ultraviolet detection at 313 nm. Under these conditions the method was reproducible with a coefficient of variation of less than 5%. The assay procedure was linear in the range 0.25-1.5 micrograms/ml, with a sensitivity of 0.01 micrograms/ml ketorolac. The recovery of ketorolac from serum ranged from 90 to 95%. |
| Databáze: | OpenAIRE |
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