Antisense therapeutics for neurofibromatosis type 1 caused by deep intronic mutations
| Autor: | Eduard Serra, Aurora Sánchez, María Asunción López-Ariztegui, Feliciano J. Ramos, Gabriel Capellá, Juana Fernández-Rodríguez, Belén Canet, Ignacio Blanco, Ana Benavides, Llúcia Benito, Eva Pros, Conxi Lázaro |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Neurofibromatosis 1 Morpholino Morpholines Population Blotting Western Gene Expression medicine.disease_cause Oligodeoxyribonucleotides Antisense Exon Genetics medicine Humans education Gene Genetics (clinical) Cells Cultured Cell Line Transformed education.field_of_study Mutation Neurofibromin 1 biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Alternative splicing Fibroblasts Molecular biology Introns nervous system diseases Alternative Splicing RNA splicing biology.protein ras Proteins RNA Splice Sites |
| Zdroj: | Human mutation. 30(3) |
| ISSN: | 1098-1004 |
| Popis: | Neurofibromatosis type 1 (NF1) is an autosomal dominant disorder affecting 1:3,500 individuals. Disease expression is highly variable and complications are diverse. However, currently there is no specific treatment for the disease. NF1 is caused by mutations in the NF1 gene, approximately 2.1% of constitutional mutations identified in our population are deep intronic mutations producing the insertion of a cryptic exon into the mature mRNA. We used antisense morpholino oligomers (AMOs) to restore normal splicing in primary fibroblast and lymphocyte cell lines derived from six NF1 patients bearing three deep intronic mutations in the NF1 gene (c.288+2025T>G, c.5749+332A>G, and c.7908-321C>G). AMOs were designed to target the newly created 5' splice sites to prevent the incorporation of cryptic exons. Our results demonstrate that AMO treatment effectively restored normal NF1 splicing at the mRNA level for the three mutations studied in the different cell lines analyzed. We also found that AMOs had a rapid effect that lasted for several days, acting in a sequence-specific manner and interfering with the splicing mechanism. Finally, to test whether the correction of aberrant NF1 splicing also restored neurofibromin function to wild-type levels, we measured the amount of Ras-GTP after AMO treatment in primary fibroblasts. The results clearly show an AMO-dependent decrease in Ras-GTP levels, which is consistent with the restoration of neurofibromin function. To our knowledge this is the first time that an antisense technique has been used successfully to correct NF1 mutations opening the possibility of a therapeutic strategy for this type of mutation not only for NF1 but for other genetic disorders. |
| Databáze: | OpenAIRE |
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