NPY modulates neurotransmission of CGRP-containing vasodilator nerves in rat mesenteric arteries
| Autor: | Koichiro Takasaki, C Nuki, Akira Saito, H. Kawasaki |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Guanethidine
Male medicine.medical_specialty Sympathetic Nervous System Physiology Calcitonin Gene-Related Peptide Cold storage Vasodilation Tetrodotoxin In Vitro Techniques Calcitonin gene-related peptide Synaptic Transmission Muscle Smooth Vascular Methoxamine chemistry.chemical_compound Physiology (medical) Internal medicine mental disorders medicine Animals Neuropeptide Y Mesenteric arteries Denervation business.industry Rats Inbred Strains Anatomy Neuropeptide Y receptor Acetylcholine Electric Stimulation Peptide Fragments humanities Mesenteric Arteries Rats medicine.anatomical_structure Endocrinology nervous system chemistry Capsaicin Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 261:H683-H690 |
| ISSN: | 1522-1539 0363-6135 |
| Popis: | The effect of neuropeptide Y (NPY) in neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves was investigated in rats. In perfused mesenteric vascular beds with active tone, perivascular nerve stimulation (PNS; 1-8 Hz) caused a frequency-dependent vasodilator response, which was abolished by 300 nM tetrodotoxin (TTX), 500 nM capsaicin, 1 microM human CGRP-(8-37), or cold storage denervation (4 degrees C for 72 h). NPY (5, 10, and 50 nM) concentration dependently inhibited the vasodilator response to PNS, whereas NPY had little effect on vasodilation induced by exogenous CGRP (10 and 100 pmol) or 1 nmol acetylcholine (ACh). NPY (10 nM) inhibited the neurogenic release of CGRP-like immunoreactivity induced by PNS (4 and 8 Hz), which was abolished by 300 nM TTX and the removal of Ca2+ from the medium. Combined perfusion with 5 nM NPY and 10 nM norepinephrine additively inhibited the vasodilator response to PNS but not to exogenous CGRP and ACh. Immunohistochemistry showed the distinct distribution of CGRP- and NPY-like immunoreactivity-containing fibers in rat mesenteric arteries. These results suggest that NPY modulates presynaptically the release of CGRP from CGRP-containing vasodilator nerves in rat mesenteric arteries. |
| Databáze: | OpenAIRE |
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