Targeted eicosanoid lipidomics of exhaled breath condensate provide a distinct pattern in the aspirin-intolerant asthma phenotype
| Autor: | Anna Gielicz, Ewa Nizankowska-Mogilnicka, Marek Sanak, Marek Kaszuba, Andrew Szczeklik, Grazyna Bochenek |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male exhaled breath condensate medicine.medical_specialty Bleeding Time Adolescent Immunology Prostaglandin Sensitivity and Specificity Gas Chromatography-Mass Spectrometry eicosanoids Drug Hypersensitivity chemistry.chemical_compound Lipoxygenase Young Adult Internal medicine Lipidomics medicine Immunology and Allergy Humans Exhaled breath condensate Child Chromatography High Pressure Liquid Asthma mass spectrometry Leukotriene Aspirin biology business.industry Middle Aged asthma medicine.disease respiratory tract diseases aspirin intolerance Endocrinology Phenotype chemistry Eicosanoid Breath Tests Exhalation Child Preschool biology.protein Eicosanoids Female business medicine.drug |
| Popis: | Background Eicosanoids, important signaling and inflammatory molecules, are present in exhaled breath condensate (EBC) in very low concentrations, requiring highly sensitive analytic methods for their quantification. Objective We sought to assess a vast platform of eicosanoids in different asthma phenotypes, including aspirin-intolerant asthma, by means of a recently developed analytic approach based on mass spectrometry. Methods EBC from 115 adult asthmatic subjects (62 with aspirin intolerance) and 38 healthy control subjects were assessed quantitatively for 19 eicosanoids by using complementary HPLC, gas chromatography–mass spectrometry, or both. Palmitic acid concentrations were used as a marker for dilution of condensate samples. Results Asthma was characterized by an increase in arachidonate lipoxygenase products and cysteinyl leukotrienes. The COX pathway was also significantly upregulated in asthmatic subjects. Subjects with aspirin-intolerant asthma were distinguished by a sharp increase in the level of prostaglandin D 2 and E 2 metabolites; their 5- and 15-hydroxyeicosateraenoic acid levels were also higher than in aspirin-tolerant subjects. A classical discriminant analysis permitted us to classify correctly 99% of asthmatic subjects within the study population; the specificity of the analysis was 97%. The eicosanoid profiling allowed for 92% correct classification of aspirin-intolerant subjects. Conclusions The highly sensitive eicosanoid profiling in EBC makes it possible to detect alterations in asthma, especially in its distinct phenotype characterized by hypersensitivity to aspirin and other nonsteroidal anti-inflammatory drugs. This permits us to discriminate asthmatic subjects from healthy subjects, as well as to distinguish the 2 asthma phenotypes based on the presence or absence of aspirin hypersensitivity. |
| Databáze: | OpenAIRE |
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