IFN-gamma facilitates NGF-induced neuronal differentiation in PC12 cells
| Autor: | G. Romeo, T. Improta, Pietro Calissano, A. Di Luzio, A.M. Salvatore, E.M. Coccia |
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| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Neurite Cell Survival Cellular differentiation Mitomycin Adrenal Gland Neoplasms Stimulation Pheochromocytoma Biology Mitomycins Interferon-gamma Mice Internal medicine medicine Tumor Cells Cultured Animals Hydroxyurea Interferon gamma Nerve Growth Factors Neurons Cell growth Cytarabine Cell Differentiation Cell Biology Recombinant Proteins Cell biology Rats Nerve growth factor Endocrinology nervous system Cell culture Colchicine medicine.drug |
| Zdroj: | Experimental cell research. 179(1) |
| ISSN: | 0014-4827 |
| Popis: | Natural or recombinant murine interferon-gamma causes a reversible arrest of proliferation of PC12 cells. Treatment with other antimitotics (AraC, colchicine, mitomycin C, hydroxyurea) or removal of serum, on the contrary, leads to mitotic arrest followed by cell death. IFN-gamma-treated PC12 cells respond more rapidly to NGF in terms of speed of neuronal outgrowth. On the other hand, NGF potentiates the action of IFN-gamma in stimulating the enzyme 2',5'-A synthetase which shifts from an average of 4.4-fold stimulation at 48 h with IFN-gamma alone to increments varying between 5- and 18-fold when PC12 cells are treated for 48 h with IFN-gamma and NGF. NGF alone, on the contrary, does not exert any detectable effect on this enzyme. From the findings we propose the use of a combined treatment of PC12 cells with NGF and IFN-gamma for a more rapid induction of neuronal differentiation. |
| Databáze: | OpenAIRE |
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