Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex-sorted Frozen-thawed Spermatozoa
| Autor: | K.M. Morton, F K Hollinshead, Sally Catt, W.M.C. Maxwell, Gareth Evans |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Sex Determination Analysis endocrine system Gestational Age Cell Separation Fertilization in Vitro Biology Cryopreservation Tissue Culture Techniques Andrology Endocrinology Embryo cryopreservation Pregnancy medicine Animals Sexual maturity Sexual Maturation Blastocyst Sheep Zygote urogenital system Pregnancy Outcome Embryo Embryo Transfer Flow Cytometry Oocyte Spermatozoa Sperm medicine.anatomical_structure Immunology Female Animal Science and Zoology Semen Preservation Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 39:454-461 |
| ISSN: | 1439-0531 0936-6768 |
| DOI: | 10.1111/j.1439-0531.2004.00542.x |
| Popis: | Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm. |
| Databáze: | OpenAIRE |
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